[Novel PCR methods for detection of HIV proviruses using digoxigenin labelled probes--Southern blot and dot blot].
作者信息
Yamaguchi K, Ushijima H, Tsuchie H, Matsumoto T, Matsuda J, Kitamura T
机构信息
Department of Internal Medicine, Juntendo University School of Medicine.
出版信息
Kansenshogaku Zasshi. 1991 Mar;65(3):267-72. doi: 10.11150/kansenshogakuzasshi1970.65.267.
Abstract
Polymerase chain reaction (PCR) is useful for diagnosis of HIV infection in the silent stage, for HIV transmission from mother to child and for existence of HIV in blood and blood products. We developed a simple, rapid and safe PCR method by using digoxigenin labelled probes. Furthermore, we developed a semi-quantitative dot blot method. These methods will be helpful for diagnosis.
摘要
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