• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

[Novel PCR methods for detection of HIV proviruses using digoxigenin labelled probes--Southern blot and dot blot].

作者信息

Yamaguchi K, Ushijima H, Tsuchie H, Matsumoto T, Matsuda J, Kitamura T

机构信息

Department of Internal Medicine, Juntendo University School of Medicine.

出版信息

Kansenshogaku Zasshi. 1991 Mar;65(3):267-72. doi: 10.11150/kansenshogakuzasshi1970.65.267.

DOI:10.11150/kansenshogakuzasshi1970.65.267
PMID:2071946
Abstract

Polymerase chain reaction (PCR) is useful for diagnosis of HIV infection in the silent stage, for HIV transmission from mother to child and for existence of HIV in blood and blood products. We developed a simple, rapid and safe PCR method by using digoxigenin labelled probes. Furthermore, we developed a semi-quantitative dot blot method. These methods will be helpful for diagnosis.

摘要

相似文献

1
[Novel PCR methods for detection of HIV proviruses using digoxigenin labelled probes--Southern blot and dot blot].
Kansenshogaku Zasshi. 1991 Mar;65(3):267-72. doi: 10.11150/kansenshogakuzasshi1970.65.267.
2
[Novel PCR methods for detection of HIV proviruses using digoxigenin labelled probes--filter paper method].
Kansenshogaku Zasshi. 1991 Mar;65(3):273-6. doi: 10.11150/kansenshogakuzasshi1970.65.273.
3
Detection of HIV1 proviral DNA by PCR and hybridization with digoxigenin labelled probes.
Mol Cell Probes. 1992 Aug;6(4):265-70. doi: 10.1016/0890-8508(92)90001-e.
4
Flow cytometric detection of human immunodeficiency virus type 1 proviral DNA by the polymerase chain reaction incorporating digoxigenin- or fluorescein-labeled dUTP.通过掺入地高辛素或荧光素标记的dUTP的聚合酶链反应,利用流式细胞术检测1型人类免疫缺陷病毒前病毒DNA。
Cytometry. 1995 Oct 1;21(2):197-202. doi: 10.1002/cyto.990210212.
5
Utilization of DNA probes with digoxigenin-modified nucleotides in southern hybridizations.
Methods Mol Biol. 1996;58:41-51. doi: 10.1385/0-89603-402-X:41.
6
Detection of African horse sickness viruses by dot-blot hybridization using a digoxigenin-labelled probe.使用地高辛标记探针通过斑点杂交法检测非洲马瘟病毒
Mol Cell Probes. 1995 Aug;9(4):233-7. doi: 10.1016/s0890-8508(95)90092-6.
7
Chemiluminescent nucleic acid detection with digoxigenin-labeled probes: a model system with probes for angiotensin converting enzyme which detect less than one attomole of target DNA.
Anal Biochem. 1991 Apr;194(1):45-53. doi: 10.1016/0003-2697(91)90149-n.
8
Rapid detection of PCR products of hepatitis C virus (HCV) with a non-radioactive oligoprobe.
New Microbiol. 1994 Jan;17(1):9-14.
9
Nonradioactive labeling and high-sensitive detection of PCR products.
Mol Biotechnol. 1994 Jun;1(3):229-40. doi: 10.1007/BF02921691.
10
[Detection of proviral HIV DNA with polymerase chain reaction in the epidermis].[采用聚合酶链反应检测表皮中的HIV前病毒DNA]
Hautarzt. 1991 Nov;42(11):704-6.