Sharer L R, Saito Y, Epstein L G, Blumberg B M
Department of Pathology, New Jersey Medical School, Newark.
Adv Neuroimmunol. 1994;4(3):283-5. doi: 10.1016/s0960-5428(06)80268-8.
In order to detect latent infection in neurons or other cell types in formalin-fixed brain tissue, we performed polymerase chain reaction amplification with incorporation of digoxigenin-conjugated deoxynucleotides, followed by in situ hybridization with biotinylated probes. The use of this two-step technique in brain tissue from a child with severe HIV-1 encephalitis revealed signal in both nuclear and perinuclear regions of cells identified as monocytes and astrocytes, and also in perineuronal satellite cells of glial morphology, but HIV-1 infection of neurons was not detected.
为了检测福尔马林固定脑组织中神经元或其他细胞类型的潜伏感染，我们进行了掺入地高辛配体偶联脱氧核苷酸的聚合酶链反应扩增，随后用生物素化探针进行原位杂交。在一名患有严重HIV-1脑炎儿童的脑组织中使用这种两步技术，在被鉴定为单核细胞和星形胶质细胞的细胞核及核周区域，以及具有神经胶质形态的神经元周围卫星细胞中均检测到信号，但未检测到神经元的HIV-1感染。
