乳菇属倍半萜类化合物及其细胞毒性研究

Lactarane sesquiterpenoids from Lactarius subvellereus and their cytotoxicity.

机构信息

Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Jangan-ku, Suwon 440-746, Republic of Korea.

出版信息

Bioorg Med Chem Lett. 2010 Sep 15;20(18):5385-8. doi: 10.1016/j.bmcl.2010.07.119. Epub 2010 Aug 3.

DOI:10.1016/j.bmcl.2010.07.119

PMID:20719504

Abstract

Subvellerolactones B (1), D (2), and E (3), structurally unusual lactarane sesquiterpenoids, were isolated from the fruiting bodies of Lactarius subvellereus together with four known lactarane sesquiterpenes (4-7). The chemical structures and stereochemistries of compounds 1-3 were determined on the basis of spectroscopic analyses, including 1D and 2D NMR experiments and a convenient Mosher ester procedure. Subvellerolactone B (1) exhibited cytotoxicity against the A549, SK-MEL-2, and HCT-15 cell lines with IC50 values of 26.5, 18.3, and 14.2 microM, respectively, and subvellerolactones D (2) and E (3) showed cytotoxicity against the A549 and HCT-15 cell lines (IC50 (2): 25.1 and 17.8 microM, and IC50 (3): 19.6 and 28.7 microM, respectively).

摘要

Subvellerolactones B (1), D (2), 和 E (3), 三种结构特殊的乳菇烷倍半萜，是从乳菇属的 subvellereus 真菌的子实体中分离得到的，同时还分离到了四个已知的乳菇烷倍半萜 (4-7)。基于光谱分析，包括 1D 和 2D NMR 实验和一个方便的 Mosher 酯法，确定了化合物 1-3 的化学结构和立体化学。Subvellerolactone B (1) 对 A549、SK-MEL-2 和 HCT-15 细胞系表现出细胞毒性，IC50 值分别为 26.5、18.3 和 14.2 μM，而 subvellerolactones D (2) 和 E (3) 对 A549 和 HCT-15 细胞系表现出细胞毒性（IC50 (2): 25.1 和 17.8 μM，和 IC50 (3): 19.6 和 28.7 μM）。