源自海洋真菌黑曲霉094102的倍半萜类化合物和苯并呋喃类化合物

Sesquiterpenoids and benzofuranoids from the marine-derived fungus Aspergillus ustus 094102.

作者信息

Lu Zhenyu, Wang Yi, Miao Chengdu, Liu Peipei, Hong Kui, Zhu Weiming

机构信息

Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, People's Republic of China.

出版信息

J Nat Prod. 2009 Oct;72(10):1761-7. doi: 10.1021/np900268z.

DOI:10.1021/np900268z

PMID:19769341

Abstract

Eight drimane sesquiterpenes (1-8), six isochromane derivatives (9-14), and three known compounds, daldinin B (15), 9alpha-hydroxy-6beta-[(2E,4E,6E)-octa-2,4,6-trienoyloxy]-5alpha-drim-7-en-11,12-olide (16), and pergillin (17), were isolated from the EtOAc extract of the marine-derived fungus Aspergillus ustus 094102. The structures of the new compounds were elucidated on the basis of spectroscopic analysis. The cytotoxic effects on A549 and HL-60 cell lines were evaluated by SRB and MTT methods. Ustusorane E (13) showed significant cytotoxicity against HL-60 cells with an IC50 value of 0.13 microM. Ustusolates C (6) and E (8) exhibited moderate cytotoxicity against A549 and HL-60 cells with IC50 values of 10.5 and 9.0 microM, respectively, and ustusolate A (4) showed weak cytotoxicity against HL-60 and A549 cells with IC50 values of 20.6 and 30.0 microM, respectively.

摘要

从海洋来源真菌黑曲霉094102的乙酸乙酯提取物中分离得到8个杜松烷倍半萜（1-8）、6个异色满衍生物（9-14）以及3个已知化合物，即daldinin B（15）、9α-羟基-6β-[(2E,4E,6E)-辛-2,4,6-三烯酰氧基]-5α-杜松-7-烯-11,12-内酯（16）和pergillin（17）。通过光谱分析阐明了新化合物的结构。采用SRB法和MTT法评估了这些化合物对A549和HL-60细胞系的细胞毒性作用。杜松烷E（13）对HL-60细胞表现出显著的细胞毒性，IC50值为0.13 μM。杜松酸酯C（6）和E（8）对A549和HL-60细胞表现出中等细胞毒性，IC50值分别为10.5和9.0 μM，而杜松酸酯A（4）对HL-60和A549细胞表现出较弱的细胞毒性，IC50值分别为20.6和30.0 μM。