Fallschissel Kerstin, Klug Kerstin, Kämpfer Peter, Jäckel Udo
Institute for Applied Microbiology, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
Ann Occup Hyg. 2010 Nov;54(8):934-43. doi: 10.1093/annhyg/meq054. Epub 2010 Aug 18.
Today's large-scale poultry production with densely stocked and enclosed production buildings is often accompanied by very high concentrations of airborne microorganisms leading to a clear health hazard for employees working in such environments. Depending on the expected exposure to microorganisms, work has to be performed under occupational safety conditions. In this study, turkey houses bioaerosols were investigated by cultivation-based and molecular methods in parallel to determine the concentrations and the composition of bacterial community. Results obtained with the molecular approach showed clearly its applicability for qualitative exposure measurements. With both, cultivation-based and molecular methods species of microorganism with a potential health risk for employees (Acinetobacter johnsonii, Aerococcus viridans, Pantoea agglomerans, and Shigella flexneri) were identified. These results underline the necessity of adequate protection measures, including the recommendation to wear breathing masks during work in poultry houses.
如今，大规模家禽养殖采用高密度饲养且禽舍封闭，常常伴随着空气中微生物浓度极高的情况，这对在此类环境中工作的员工构成了明显的健康危害。根据预期的微生物暴露情况，必须在职业安全条件下开展工作。在本研究中，通过基于培养的方法和分子方法并行研究火鸡舍中的生物气溶胶，以确定细菌群落的浓度和组成。分子方法获得的结果清楚地表明了其在定性暴露测量中的适用性。通过基于培养的方法和分子方法，均鉴定出了对员工有潜在健康风险的微生物种类（约翰逊不动杆菌、绿色气球菌、成团泛菌和福氏志贺菌）。这些结果强调了采取适当保护措施的必要性，包括建议在禽舍工作时佩戴呼吸面罩。
