Terai K, Yoshida K, Yoshiike M, Fujime M, Iwamoto T
Department of Urology, St. Marianna University School of Medicine, Kawasaki, Japan.
Urol Int. 2010;85(2):209-15. doi: 10.1159/000315976. Epub 2010 Aug 18.
Seminal plasma motility inhibitors (SPMIs) are proteinase-resistant fragments of semenogelin I and II (Sgs), which are the major proteins of semen coagulum. SPMIs inhibit the motility of spermatozoa, and Sgs are thought to be natural regulators of human sperm function. The mechanism underlying sperm motility regulation and its association with defective motility in infertile men remain unclear. The purpose of this study was to investigate the association between SPMIs and spermatozoa in infertile men with asthenozoospermia. Fifty-four semen samples from 37 asthenozoospermic patients and 17 samples from 9 normal healthy subjects were analyzed. Spermatozoa, washed by Percoll density gradients, were immunostained with anti-SPMI antibody and subjected to flow cytometric analysis. The proportion of spermatozoa labeled with the antibody and the average intensity of fluorescence labeling per spermatozoa were analyzed in relation to the parameters used for semen analysis. A significant negative correlation was found between sperm motility and the proportion (R = -0.68) and intensity (R = -0.38) of labeling. These results suggest that SPMIs remain on the sperm surface after liquefaction. This might account for some disorders of sperm motility observed in infertile men with asthenozoospermia.
精浆运动抑制剂（SPMIs）是精液凝块主要蛋白质精液凝固蛋白I和II（Sgs）的抗蛋白酶片段。SPMIs抑制精子的运动，并且Sgs被认为是人类精子功能的天然调节剂。精子运动调节的潜在机制及其与不育男性运动缺陷的关联仍不清楚。本研究的目的是调查弱精子症不育男性中SPMIs与精子之间的关联。分析了来自37名弱精子症患者的54份精液样本和来自9名正常健康受试者的17份样本。通过Percoll密度梯度洗涤的精子用抗SPMI抗体进行免疫染色，并进行流式细胞术分析。根据用于精液分析的参数，分析了用抗体标记的精子比例和每个精子的平均荧光标记强度。发现精子活力与标记比例（R = -0.68）和强度（R = -0.38）之间存在显著负相关。这些结果表明，液化后SPMIs仍保留在精子表面。这可能解释了在弱精子症不育男性中观察到的一些精子运动障碍。
