Aboutorabi Roshanak, Mazani Fatemeh, Rafiee Laleh
Head of Infertility Laboratory, Shahid Beheshti Hospital, Isfahan University of Medical Sciences, Isfahan, Iran ; Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2014 May 28;3:124. doi: 10.4103/2277-9175.133201. eCollection 2014.
Sperm maturation and sperm membrane integration are the most important elements in male fertility. CD52 is one of the antigens. CD52 is a GPI (glycosylphosphatidylinositol) anchored that express on lymphocytes and epididymal cells. This antigen bind to sperm membrane during transition sperm from epididymal duct as well as its relationship with semenogelins in human seminal plasma. The aim of this study was to obtain any association between the percentage of CD52 positive sperms with main semen parameters such as percentage of motile sperms, percentage of sperm with normal morphology, and the presence of normal viscosity.
Semen samples from subfertile men were analyzed, the samples totally were 45 that divided according to their motility into three groups, first one, more than 40%, second one 10-40%, and the third one under 10% total motility. Fifteen samples in each group were evaluated by semen analysis according to WHO 2010 guidelines for infertility laboratory. Sperms were washed by Ham's F-10 and immunostaining with the monoclonal antibody CAMPATH-1G and then analyzed by flow cytometry. We compared each of the groups based on their motility and the data were analyzed by SPSS 20.
Correlation between CD52 labeling and sperm motility was negatively significant, in the second group (r = -0.592, P = 0.020) and in the third group (r = -0.805, P = 0.00).
Our results showed that the correlation between CD52 labeling and sperm motility was negatively significant, but we did not observe any relation with other semen parameters, such as sperm normal morphology, sperm concentration, and semen viscosity.
精子成熟和精子膜整合是男性生育能力的最重要因素。CD52是其中一种抗原。CD52是一种糖基磷脂酰肌醇（GPI）锚定蛋白，表达于淋巴细胞和附睾细胞。这种抗原在精子从附睾管过渡期间与精子膜结合，以及它与人类精浆中精液凝胶蛋白的关系。本研究的目的是获取CD52阳性精子百分比与主要精液参数之间的任何关联，如活动精子百分比、正常形态精子百分比以及正常粘度的存在情况。
对不育男性的精液样本进行分析，样本共45份，根据其活力分为三组，第一组，活力超过40%，第二组10 - 40%，第三组总活力低于10%。根据世界卫生组织2010年不育实验室指南，对每组15个样本进行精液分析评估。精子用哈姆氏F - 10洗涤，并用单克隆抗体CAMPATH - 1G进行免疫染色，然后通过流式细胞术进行分析。我们根据活力对每组进行比较，并使用SPSS 20对数据进行分析。
CD52标记与精子活力之间的相关性呈负显著，在第二组（r = -0.592，P = 0.020）和第三组（r = -0.805，P = 0.00）。
我们的结果表明，CD52标记与精子活力之间的相关性呈负显著，但我们未观察到与其他精液参数有任何关系，如精子正常形态、精子浓度和精液粘度。
