Molecular Gastroenterology G350, DKFZ Heidelberg, Heidelberg, Germany.
Pancreatology. 2010;10(4):462-6. doi: 10.1159/000264677. Epub 2010 Aug 19.
Helicobacter pylori has been suggested to be involved in pancreatic diseases, namely autoimmune pancreatitis and pancreatic carcinoma. We investigated the presence of conserved sequences of Helicobacter in pancreatic tissue and pancreatic juice from patients with chronic nonautoimmune and autoimmune pancreatitis as well as pancreatic ductal adenocarcinoma (PDAC).
35 pancreatic juices collected during routine endoscopic retrograde cholangiopancreatography and 30 pancreatic tissues were studied. Nested PCR was used to detect H. pylori in the isolated DNA samples. In order to exclude a methodological bias, the samples were analyzed blindly in 2 different laboratories using either conventional or LightCycler PCR for H. pylori urease A and 16S ribosomal DNA.
In the pancreas of 11 patients with autoimmune pancreatitis, no H. pylori DNA could be detected. Further, in none of the other tissue samples of chronic pancreatitis or PDAC could we detect any Helicobacter sequences. Out of the pancreatic juice samples, none demonstrated either of the 2 Helicobacter gene sequences investigated.
Despite good scientific reasoning for an involvement of Helicobacter in pancreatic diseases, a direct infection of the microbial agent seems unlikely. Rather, the pathomechanism must involve molecular mimicry in autoimmune pancreatitis, or the transformation of nitric food constituents to nitrosamines in pancreatic cancer. and IAP.
幽门螺杆菌被认为与胰腺疾病有关，即自身免疫性胰腺炎和胰腺癌。我们研究了慢性非自身免疫性和自身免疫性胰腺炎以及胰腺导管腺癌（PDAC）患者胰腺组织和胰液中幽门螺杆菌保守序列的存在。
在常规内镜逆行胰胆管造影期间收集了 35 份胰液和 30 份胰腺组织。巢式 PCR 用于检测分离的 DNA 样本中的幽门螺杆菌。为了排除方法学偏见，使用常规或 LightCycler PCR 分析了来自 2 个不同实验室的样本，用于检测 H. pylori urease A 和 16S 核糖体 DNA。
在 11 例自身免疫性胰腺炎患者的胰腺中未检测到 H. pylori DNA。此外，在慢性胰腺炎或 PDAC 的其他组织样本中，我们均未检测到任何幽门螺杆菌序列。在胰液样本中，没有一个样本显示出所研究的 2 种幽门螺杆菌基因序列之一。
尽管有充分的科学依据表明幽门螺杆菌与胰腺疾病有关，但直接感染这种微生物似乎不太可能。相反，在自身免疫性胰腺炎中，发病机制必须涉及分子模拟，或者在胰腺癌和 IAP 中，将硝酸盐食物成分转化为亚硝胺。
