幽门螺杆菌与自身免疫性胰腺炎和胰腺癌。

Helicobacter pylori in autoimmune pancreatitis and pancreatic carcinoma.

机构信息

Molecular Gastroenterology G350, DKFZ Heidelberg, Heidelberg, Germany.

出版信息

Pancreatology. 2010;10(4):462-6. doi: 10.1159/000264677. Epub 2010 Aug 19.

DOI:10.1159/000264677

PMID:20720447

Abstract

BACKGROUND

Helicobacter pylori has been suggested to be involved in pancreatic diseases, namely autoimmune pancreatitis and pancreatic carcinoma. We investigated the presence of conserved sequences of Helicobacter in pancreatic tissue and pancreatic juice from patients with chronic nonautoimmune and autoimmune pancreatitis as well as pancreatic ductal adenocarcinoma (PDAC).

METHODS

35 pancreatic juices collected during routine endoscopic retrograde cholangiopancreatography and 30 pancreatic tissues were studied. Nested PCR was used to detect H. pylori in the isolated DNA samples. In order to exclude a methodological bias, the samples were analyzed blindly in 2 different laboratories using either conventional or LightCycler PCR for H. pylori urease A and 16S ribosomal DNA.

RESULTS

In the pancreas of 11 patients with autoimmune pancreatitis, no H. pylori DNA could be detected. Further, in none of the other tissue samples of chronic pancreatitis or PDAC could we detect any Helicobacter sequences. Out of the pancreatic juice samples, none demonstrated either of the 2 Helicobacter gene sequences investigated.

CONCLUSION

Despite good scientific reasoning for an involvement of Helicobacter in pancreatic diseases, a direct infection of the microbial agent seems unlikely. Rather, the pathomechanism must involve molecular mimicry in autoimmune pancreatitis, or the transformation of nitric food constituents to nitrosamines in pancreatic cancer. and IAP.

摘要

背景

幽门螺杆菌被认为与胰腺疾病有关，即自身免疫性胰腺炎和胰腺癌。我们研究了慢性非自身免疫性和自身免疫性胰腺炎以及胰腺导管腺癌（PDAC）患者胰腺组织和胰液中幽门螺杆菌保守序列的存在。

方法

在常规内镜逆行胰胆管造影期间收集了 35 份胰液和 30 份胰腺组织。巢式 PCR 用于检测分离的 DNA 样本中的幽门螺杆菌。为了排除方法学偏见，使用常规或 LightCycler PCR 分析了来自 2 个不同实验室的样本，用于检测 H. pylori urease A 和 16S 核糖体 DNA。