Winkler Robert, García-Lara Silverio
Department of Biotechnology and Food Engineering, Tecnológico de Monterrey, ITESM-Campus Monterrey, Eugenio Garza Sada 2501, CP 64849, Monterrey, NL, Mexico.
Mol Biosyst. 2010 Oct;6(10):1810-2. doi: 10.1039/c003112g. Epub 2010 Aug 19.
Maize grain peroxidases are associated with insect resistance. In this study we present an activity directed approach for the identification of peroxidases localized in different grain tissues. With drastically reduced proteomic effort we could identify different peroxidases for maize weevil susceptible and resistant genotypes of maize. Our strategy also allows for the identification of previously unknown plant peroxidases.
玉米籽粒过氧化物酶与抗虫性有关。在本研究中，我们提出了一种基于活性导向的方法来鉴定定位在不同籽粒组织中的过氧化物酶。通过大幅减少蛋白质组学研究工作量，我们能够鉴定出玉米象易感和抗性玉米基因型中的不同过氧化物酶。我们的策略还能够鉴定出以前未知的植物过氧化物酶。
