López-Castillo L Margarita, González-Leyzaola Alán, Diaz-Flores-Rivera M Fernanda, Winkler Robert, Wielsch Natalie, García-Lara Silverio
School of Engineering and Sciences, Tecnologico de Monterrey, Monterrey, Mexico.
Department of Biotechnology and Biochemistry, CINVESTAV Unidad Irapuato, Guanajuato, Mexico.
Front Plant Sci. 2020 Jun 11;11:781. doi: 10.3389/fpls.2020.00781. eCollection 2020.
Peroxidases (PODs) have many biological functions during the plant life cycle. In maize kernels, endosperm PODs have been identified as biochemical contributors to resistance against , but their identities have not been determined. In this study, we identified these PODs and determined whether their contributions are basal or inducible. Semi-purification and LC-MS/MS analyses showed that the protein ZmPrx35 is the predominant soluble endosperm POD from kernels of Pob84-C3R. Subsequent time-course analyses after mechanical damage showed that POD activity was regulated in a fluctuating kinetics pattern and that mRNA expression levels reflected this pattern. After 48 h of infestation with or , soluble endosperm POD activities were 1.38- or 0.85-fold, respectively. Under the same conditions, expression was induced 1.61-fold ( infestation) and 1.17-fold ( infestation). These findings suggest that ZmPrx35 contributes to the protective responses of aleurone cells against wounding and pest attacks, which could be enhanced/repressed by insect factors. Our data also provide evidence that the mechanisms of resistance of maize Pob84-C3R kernels toward the insect pests and are independent.
过氧化物酶（PODs）在植物生命周期中具有多种生物学功能。在玉米籽粒中，胚乳PODs已被确定为抗[具体害虫名称未给出]的生化贡献者，但其具体身份尚未确定。在本研究中，我们鉴定了这些PODs，并确定它们的贡献是基础的还是可诱导的。半纯化和液相色谱-串联质谱分析表明，蛋白ZmPrx35是来自Pob84-C3R籽粒的主要可溶性胚乳POD。机械损伤后的后续时间进程分析表明，POD活性以波动的动力学模式调节，且mRNA表达水平反映了这种模式。用[具体害虫名称未给出]或[具体害虫名称未给出]侵染48小时后，可溶性胚乳POD活性分别为1.38倍或0.85倍。在相同条件下，ZmPrx35表达在[具体害虫名称未给出]侵染时诱导1.61倍，在[具体害虫名称未给出]侵染时诱导1.17倍。这些发现表明，ZmPrx35有助于糊粉层细胞对伤口和害虫攻击的保护反应，这可能会被昆虫因子增强/抑制。我们的数据还提供了证据，表明玉米Pob84-C3R籽粒对害虫[具体害虫名称未给出]和[具体害虫名称未给出]的抗性机制是独立的。
