Briantseva S A, Gavriushina E S, Emets A I, Karpov P A, Blium Ia B, Drygin Iu F, Nadezhdina E S
Tsitol Genet. 2010 Jul-Aug;44(4):41-7.
The aim of our work is the identification of protein kinases phosphorylating microtubule proteins in plant cells. Using bioinformatic approach, we found genes of putative homologues of microtubule-associated mammalian protein kinase MAST2 in higher plant genomes. The gene of closest MAST2 homologue, putative protein, named GMLK (Grape MAST2-Like Kinase, A7NTE9_VITVI), was found in grape Vitis vinifera. We report here the cloning of cDNA of GMLK (A7NTE9) from Pinot Noir grape vine leaves.
我们工作的目的是鉴定植物细胞中使微管蛋白磷酸化的蛋白激酶。利用生物信息学方法，我们在高等植物基因组中发现了与微管相关的哺乳动物蛋白激酶MAST2推定同源物的基因。在葡萄（Vitis vinifera）中发现了最接近MAST2同源物的基因，其推定蛋白名为GMLK（葡萄MAST2样激酶，A7NTE9_VITVI）。我们在此报告从黑比诺葡萄叶片中克隆GMLK（A7NTE9）的cDNA。
