Institute of Protein Research of Russian Academy of Sciences, Moscow, Russia.
Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kiev, Ukraine.
Planta. 2017 Nov;246(5):959-969. doi: 10.1007/s00425-017-2742-4. Epub 2017 Jul 17.
The similarity of IREH1 (Incomplete Root Hair Elongation 1) and animal MAST kinases was confirmed; IREH1cDNA was cloned while expressing in cultured animal cells co-localized with the centrosome. In mammals and fruit flies, microtubule-associated serine/threonine-protein kinases (MAST) are strongly involved in the regulation of the microtubule system. Higher plants also possess protein kinases homologous to MASTs, but their function and interaction with the cytoskeleton remain unclear. Here, we confirmed the sequence and structural similarity of MAST-related putative protein kinase IREH1 (At3g17850) and known animal MAST kinases. We report the first cloning of full-length cDNA of the IREH1 from Arabidopsis thaliana. Recombinant GFP-IREH1 protein was expressed in different cultured animal cells. It revealed co-localization with the centrosome without influencing cell morphology and microtubule arrangement. Structural N-terminal region of the IREH1 molecule co-localized with centrosome as well.
IREH1（不完全根毛伸长 1）与动物 MAST 激酶的相似性得到了确认；在培养的动物细胞中表达时，克隆了 IREH1 cDNA，与中心体共定位。在哺乳动物和果蝇中，微管相关丝氨酸/苏氨酸蛋白激酶（MAST）强烈参与微管系统的调节。高等植物也具有与 MAST 同源的蛋白激酶，但它们的功能及其与细胞骨架的相互作用尚不清楚。在这里，我们证实了与已知动物 MAST 激酶具有序列和结构相似性的 MAST 相关假定蛋白激酶 IREH1（At3g17850）。我们首次从拟南芥中克隆了全长 cDNA 的 IREH1。重组 GFP-IREH1 蛋白在不同的培养动物细胞中表达。结果表明，它与中心体共定位，而不影响细胞形态和微管排列。IREH1 分子的结构 N 端区域也与中心体共定位。
