Biology of Reproduction Research Unit, Physiology of Reproduction Laboratory FES Zaragoza, UNAM, México, DF, México.
Reprod Biol Endocrinol. 2010 Aug 19;8:99. doi: 10.1186/1477-7827-8-99.
The present study tested the hypothesis that if polycystic ovary syndrome (PCOS) results from activating the noradrenergic outflow to the ovary, unilaterally sectioning the superior ovarian nerve (SON) will result in ovulation by the denervated ovary, and the restoration of progesterone (P4), testosterone (T) and estradiol (E2) normal serum level. A single 2 mg dose of estradiol valerate (EV) to adult rats results in the development of a syndrome similar to the human PCOS. Ten-day old rats were injected with EV or vehicle solution (Vh) and were submitted to sham surgery, unilateral or bilateral sectioning of the SON at 24-days of age. The animals were sacrificed at 90 to 92 days of age, when they presented vaginal estrus preceded by a pro-estrus smear. In EV-treated animals, unilateral sectioning of the SON restored ovulation by the innervated ovary and unilateral or bilateral sectioning of the SON normalized testosterone and estradiol levels. These results suggest that aside from an increase in ovarian noradrenergic tone in the ovaries, in the pathogenesis of the PCOS participate other neural influences arriving to the ovaries via the SON, regulating spontaneous ovulation. Changes in P4, T and E2 serum levels induced by EV treatment seem to be controlled by neural signals arising from the abdominal wall and other signals arriving to the ovaries through the SON, and presents asymmetry.
如果多囊卵巢综合征（PCOS）是由去甲肾上腺素能神经向卵巢的传出激活引起的，那么单侧切断卵巢上神经（SON）将导致去神经卵巢排卵，并恢复孕激素（P4）、睾酮（T）和雌二醇（E2）的正常血清水平。单次 2 毫克戊酸雌二醇（EV）注射成年大鼠可导致类似于人类 PCOS 的综合征。在 10 天大的大鼠中注射 EV 或载体溶液（Vh），并在 24 天大时进行假手术、单侧或双侧切断 SON。动物在 90 至 92 天大时被处死，此时它们出现阴道发情，之前有发情前涂片。在 EV 处理的动物中，单侧切断 SON 恢复了神经支配卵巢的排卵，单侧或双侧切断 SON 使睾酮和雌二醇水平正常化。这些结果表明，除了卵巢去甲肾上腺素能张力增加外，其他通过 SON 到达卵巢的神经影响也参与了 PCOS 的发病机制，调节自发性排卵。EV 处理引起的 P4、T 和 E2 血清水平的变化似乎受到来自腹壁的神经信号和通过 SON 到达卵巢的其他信号的控制，并且存在不对称性。
