Department of Obstetrics and Gynecology, University Hospital, Berne, Switzerland.
Am J Obstet Gynecol. 2010 Oct;203(4):399.e1-7. doi: 10.1016/j.ajog.2010.06.054. Epub 2010 Aug 17.
Preeclampsia is associated with perinatal brain injury. Autologous placenta stem cell transplantation represents a promising future treatment option for neuroregeneration. The aim of this study was to compare the neuroregenerative capacity of preeclampsia-placenta stem cells to previously characterized placentas from uncomplicated pregnancies.
Placenta stem cells from amnion (epithelium, mesenchyme) and chorion were assessed for cell surface markers and the formation of neuronal-like cells, oligodendrocytes and their progenitors in culture.
Markers of preeclampsia-placenta stem cells were different from uncomplicated pregnancies-placenta stem cells in amnion epithelium and chorion, but not in amnion mesenchyme. Similarly to uncomplicated pregnancies-placenta stem cells, preeclampsia-placenta stem cells derived from amnion and chorion differentiated preferably into nestin-positive stem/progenitor cells and Tuj-1-positive neurons. However, other important markers were varying after neurogenic differentiation of uncomplicated pregnancies- and preeclampsia-placenta stem cells.
Surface marker expression patterns of preeclampsia-placenta stem cell's and uncomplicated pregnancies-placenta stem cell's differ. In vitro differentiation assays, however, provide evidence that both preeclampsia-placenta stem cells and uncomplicated pregnancies-placenta stem cells are comparably suitable for neuroregeneration purposes.
子痫前期与围产期脑损伤有关。自体胎盘干细胞移植为神经再生提供了一种很有前途的未来治疗选择。本研究旨在比较子痫前期胎盘干细胞与以前从无并发症妊娠中所鉴定的胎盘的神经再生能力。
从羊膜（上皮、间充质）和绒毛膜评估胎盘干细胞的细胞表面标志物，并在培养中形成神经元样细胞、少突胶质细胞及其前体细胞。
子痫前期胎盘干细胞的标志物与无并发症妊娠胎盘干细胞在羊膜上皮和绒毛膜中不同，但在羊膜间充质中没有不同。与无并发症妊娠胎盘干细胞一样，来源于羊膜和绒毛膜的子痫前期胎盘干细胞优先分化为巢蛋白阳性的干细胞/祖细胞和 Tuj-1 阳性神经元。然而，无并发症妊娠和子痫前期胎盘干细胞的神经发生分化后，其他重要标志物也在变化。
子痫前期胎盘干细胞和无并发症妊娠胎盘干细胞的表面标志物表达模式不同。然而，体外分化实验提供了证据，表明子痫前期胎盘干细胞和无并发症妊娠胎盘干细胞都适合神经再生目的。
