Department of Materials Science and Engineering, The Ohio State University, Columbus, OH 43210, USA.
J Biomech. 2010 Dec 1;43(16):3183-90. doi: 10.1016/j.jbiomech.2010.07.026. Epub 2010 Aug 17.
Engineered skin must be mechanically strong to facilitate surgical application and prevent damage during the early stages of engraftment. However, the evolution of structural properties during culture, the relative contributions of the epidermis and dermis, and any correlation with tissue morphogenesis are not well known. These aspects are investigated by assessing the mechanical properties of engineered skin (ES) and engineered dermis (ED) during a 21-day culture period, including correlations with cellular metabolism, cellular organization and epidermal differentiation. During culture, the epidermis differentiates and begins to cornify, as evidenced by immunostaining and surface electrical capacitance. Tensile testing reveals that the ultimate tensile strength and linear stiffness increase linearly with time for ES, but are relatively unchanged for ED. ES strength correlates significantly with epidermal differentiation (p < 0.001) and a composite strength model indicates that strength is largely determined by the epidermis. These data suggest that strategies to improve ES biomechanics should target the dermis. Additionally, time-dependant changes in average ES strength and percent elongation can be used to set upper bound limits on mechanical stimulation profiles to avoid tissue damage.
工程化皮肤必须具有机械强度，以方便手术应用并防止在植入的早期阶段受损。然而，在培养过程中结构性能的演变、表皮和真皮的相对贡献，以及与组织形态发生的任何相关性尚不清楚。通过评估工程化皮肤（ES）和工程化真皮（ED）在 21 天培养期间的机械性能，包括与细胞代谢、细胞组织和表皮分化的相关性，研究了这些方面。在培养过程中，表皮分化并开始角化，这可以通过免疫染色和表面电容来证明。拉伸测试表明，ES 的极限拉伸强度和线性刚度随时间呈线性增加，但 ED 则相对不变。ES 的强度与表皮分化显著相关（p < 0.001），并且复合强度模型表明强度主要由表皮决定。这些数据表明，改善 ES 生物力学的策略应针对真皮。此外，平均 ES 强度和伸长率的时间依赖性变化可用于设置机械刺激曲线的上限限制，以避免组织损伤。
