Blackstone Britani Nicole, Drexler Jason William, Powell Heather Megan
1 Department of Biomedical Engineering, The Ohio State University , Columbus, Ohio.
Tissue Eng Part A. 2014 Oct;20(19-20):2746-55. doi: 10.1089/ten.TEA.2013.0687. Epub 2014 May 20.
Autologous engineered skin (ES) offers promise as a treatment for massive full thickness burns. Unfortunately, ES is orders of magnitude weaker than normal human skin causing it to be difficult to apply surgically and subject to damage by mechanical shear in the early phases of engraftment. In addition, no manufacturing strategy has been developed to tune ES biomechanics to approximate the native biomechanics at different anatomic locations. To enhance and tune ES biomechanics, a coaxial (CoA) electrospun scaffold platform was developed from polycaprolactone (PCL, core) and gelatin (shell). The ability of the coaxial fiber core diameter to control both scaffold and tissue mechanics was investigated along with the ability of the gelatin shell to facilitate cell adhesion and skin development compared to pure gelatin, pure PCL, and a gelatin-PCL blended fiber scaffold. CoA ES exhibited increased cellular adhesion and metabolism versus PCL alone or gelatin-PCL blend and promoted the development of well stratified skin with a dense dermal layer and a differentiated epidermal layer. Biomechanics of the scaffold and ES scaled linearly with core diameter suggesting that this scaffold platform could be utilized to tailor ES mechanics for their intended grafting site and reduce graft damage in vitro and in vivo.
自体工程皮肤（ES）有望成为大面积全层烧伤的一种治疗方法。不幸的是，ES的强度比正常人类皮肤弱几个数量级，这使得其在手术应用时较为困难，并且在植入的早期阶段容易受到机械剪切力的损伤。此外，尚未开发出能够调整ES生物力学以使其接近不同解剖位置天然生物力学的制造策略。为了增强和调整ES的生物力学性能，开发了一种由聚己内酯（PCL，芯层）和明胶（壳层）组成的同轴（CoA）电纺支架平台。研究了同轴纤维芯层直径控制支架和组织力学性能的能力，以及与纯明胶、纯PCL和明胶-PCL混合纤维支架相比，明胶壳层促进细胞黏附和皮肤发育的能力。与单独的PCL或明胶-PCL混合物相比，CoA ES表现出更高的细胞黏附和代谢水平，并促进了具有致密真皮层和分化表皮层的分层良好的皮肤的发育。支架和ES的生物力学性能与芯层直径呈线性比例关系，这表明该支架平台可用于根据预期的移植部位定制ES的力学性能，并减少体内外移植损伤。
