在人类免疫缺陷病毒内切酶蛋白中鉴定出一个独特的免疫显性交叉反应位点。

Identification of a uniquely immunodominant, cross-reacting site in the human immunodeficiency virus endonuclease protein.

作者信息

Björling E, Utter G, Stålhandske P, Norrby E, Chiodi F

机构信息

Department of Virology, Karolinska Institute, Stockholm, Sweden.

出版信息

J Virol. 1991 Aug;65(8):4543-6. doi: 10.1128/JVI.65.8.4543-4546.1991.

DOI:10.1128/JVI.65.8.4543-4546.1991

PMID:2072463

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC248902/

Abstract

One of the features of the life cycle of retroviruses is insertion of the proviral DNA into host chromosomes. A protein encoded by the 3' end of the pol gene of the virus genome has been shown to possess endonuclease activity (D. P. Grandgenett, A. C. Vora, and R. D. Schiff, Virology 89:119-132, 1978), which is necessary for DNA integration. Sera from the majority of human immunodeficiency virus (HIV)-infected individuals react with endonuclease protein p31 in serological tests (J. S. Allan, J. E. Coligan, T.-H. Lee, F. Barin, P. J. Kanki, S. M'Boup, M. F. McLane, J. E. Groopman, and M. Essex, Blood 69:331-333, 1987; E. F. Lillehoj, F. H. R. Salazar, R. J. Mervis, M. G. Raum, H. W. Chan, N. Ahmad, and S. Venkatesan, J. Virol. 62:3053-3058, 1988; K. S. Steimer, K. W. Higgins, M. A. Powers, J. C. Stephans, A. Gyenes, G. George-Nascimento, P. A. Liciw, P. J. Barr, R. A. Hallewell, and R. Sanchez-Pescador, J. Virol. 58:9-16, 1986). It is not known, however, which part of the protein represents the target(s) for antibody response. To study this, we synthesized peptides and used them in an enzyme-linked immunosorbent assay system to map the reactivity of human immunodeficiency virus type 1 (HIV-1) antibody-positive sera to the different regions of the HIV endonuclease. A uniquely antigenic, HIV-1- and HIV-2-cross-reacting site was identified in the central part of this protein from Phe-663 to Trp-670.

摘要

逆转录病毒生命周期的特征之一是将前病毒DNA插入宿主染色体。病毒基因组pol基因3'端编码的一种蛋白质已被证明具有内切核酸酶活性（D.P.格兰德根内特、A.C.沃拉和R.D.希夫，《病毒学》89:119 - 132，1978），这是DNA整合所必需的。在血清学检测中，大多数人类免疫缺陷病毒（HIV）感染个体的血清与内切核酸酶蛋白p31发生反应（J.S.艾伦、J.E.科利根、T.-H.李、F.巴林、P.J.坎基、S.姆布普、M.F.麦克莱恩、J.E.格鲁普曼和M.埃塞克斯，《血液》69:331 - 333，1987；E.F.利勒霍伊、F.H.R.萨拉扎、R.J.默维斯、M.G.劳姆、H.W.陈、N.艾哈迈德和S.文卡特桑，《病毒学杂志》62:3053 - 3058，1988；K.S.施泰默、K.W.希金斯、M.A.鲍尔斯、J.C.斯蒂芬斯、A.吉耶内斯、G.乔治 - 纳西索、P.A.利西夫、P.J.巴尔、R.A.哈勒韦尔和R.桑切斯 - 佩斯卡多，《病毒学杂志》58:9 - 16，1986）。然而，尚不清楚该蛋白质的哪一部分是抗体反应的靶点。为了研究这一点，我们合成了肽段，并将它们用于酶联免疫吸附测定系统，以绘制1型人类免疫缺陷病毒（HIV - 1）抗体阳性血清与HIV内切核酸酶不同区域的反应性图谱。在该蛋白质从苯丙氨酸 - 663到色氨酸 - 670的中央部分鉴定出一个独特的抗原性、HIV - 1和HIV - 2交叉反应位点。