Wang Dong-yan, Zhang Yong, Zhu Shuang-li
World Health Organization Regional Office for the Western Pacific Regional Reference Poliovirus Laboratory, State Key Laboratory Molecular Virology & Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zhongguo Yi Miao He Mian Yi. 2010 Jun;16(3):197-202, 218.
To identify and describe the genetic characteristics of non-polio enteroviruses (NPEVs) which could multiplicated on L20B cell line, and discuss the impact to isolation rate of polioviruses.
5 L20B positive isolates were collected from stool samples of patient with acute flaccid paralysis (AFP) and health children in Guangxi Zhuang autonomous region and Ningxia Hui autonomous region. These isolates were analysed using VP4 nucleotide sequencing method. The completed VP, coding regions were amplified and sequenced. Finally, phylogenetic tree were constructed base on completed VP1 coding regions sequences of these L20B positive isolates and those downloaded form GenBank database.
The 5 NPEVs were all identified as coxsackieviruses group A (CVA). Among them, 1 was identified as CVA4, 1 was identified as CVA8 and 3 were identified as CVA10, and all of them belonged to human enterovirus species A (HEV-A). Nucleotide homology analysis and phylogenetic analysis base on completed VP1 coding regions sequences showed that all these 3 serotypes of NPEVs clustered independently, and they were all native circulated viruses in mainland China.
5 NPEVs in this study belonged to HEV-A, covered 3 serotypes, CVA4, CVA8 and CVA10. Due to the high sensitivity of primarily inoculation of poliovirus onto L20B cell, the isolation rate of polioviruses could not be affected so much. Hand, foot, and mouth disease (HFMD) outbreaks in mainland China in recent years, and it was found that HEV-A is also pathogen of HFMD. Identification of these viruses have current significance to differential diagnosis of poliovirus infection and HFMD control and prevention.
鉴定并描述可在L20B细胞系上增殖的非脊髓灰质炎肠道病毒（NPEV）的基因特征，并探讨其对脊髓灰质炎病毒分离率的影响。
从广西壮族自治区和宁夏回族自治区急性弛缓性麻痹（AFP）患者及健康儿童的粪便样本中收集5株L20B阳性分离株。采用VP4核苷酸测序法对这些分离株进行分析。扩增并测序完整的VP编码区。最后，基于这些L20B阳性分离株以及从GenBank数据库下载的完整VP1编码区序列构建系统发育树。
5株NPEV均被鉴定为A组柯萨奇病毒（CVA）。其中，1株被鉴定为CVA4，1株被鉴定为CVA8，3株被鉴定为CVA10，它们均属于人肠道病毒A种（HEV-A）。基于完整VP1编码区序列的核苷酸同源性分析和系统发育分析表明，这3种血清型的NPEV均独立聚类，且均为中国大陆本土流行病毒。
本研究中的5株NPEV属于HEV-A，涵盖CVA4、CVA8和CVA10这3种血清型。由于脊髓灰质炎病毒初次接种到L20B细胞上的敏感性较高，脊髓灰质炎病毒的分离率不会受到太大影响。近年来中国大陆手足口病（HFMD）疫情爆发，发现HEV-A也是HFMD的病原体。鉴定这些病毒对脊髓灰质炎病毒感染的鉴别诊断以及HFMD的防控具有现实意义。
