Unitat de Bioquímica i Biologia Molecular, Departament de Biologia, Universitat de Girona, Girona, Spain.
OMICS. 2010 Aug;14(4):465-74. doi: 10.1089/omi.2010.0050.
Prostate-specific antigen (PSA) two-dimensional electrophoresis (2-DE) subforms (F1-F5) have been described to be altered in prostate cancer (PCa) compared to benign prostatic hyperplasia (BPH). To understand their molecular differences, characterization of these subforms from PCa serum and seminal plasma, namely, at the glycan level, was performed. PSA 2-DE subforms from two serum PCa samples and seminal plasma were analyzed by N-glycan sequencing using high-performance liquid chromatography (HPLC) combined with exoglycosidase array digestions and by mass spectrometry. F1, F2, and F3 subforms showed the same N-glycan pattern, which contained higher levels of sialic acid than the F4 subform, whereas the F5 subform was unglycosylated. When comparing PSA subforms from PCa with seminal plasma, a decrease in sialylation was observed. Furthermore, the analysis of F3, the more abundant PSA subform, showed a higher proportion of alpha 2-3 sialic acid and a decrease in core fucosylated glycans in the PCa sample. These N-glycan changes in PCa PSA subforms highlight the importance of glycosylation as an indicator of PCa disease.
前列腺特异性抗原（PSA）二维电泳（2-DE）亚基（F1-F5）在前列腺癌（PCa）中与良性前列腺增生（BPH）相比发生了改变。为了了解它们的分子差异，对来自 PCa 血清和精液的这些亚基进行了糖基化水平的特征分析。使用高效液相色谱（HPLC）结合外切糖苷酶阵列消化和质谱法对来自两个血清 PCa 样本和精液的 PSA 2-DE 亚基进行了 N-糖测序分析。F1、F2 和 F3 亚基表现出相同的 N-聚糖模式，其唾液酸水平高于 F4 亚基，而 F5 亚基未糖基化。当比较 PCa 与精液中的 PSA 亚基时，观察到唾液酸化程度降低。此外，对更丰富的 PSA 亚基 F3 的分析显示，PCa 样本中 alpha 2-3 唾液酸的比例更高，核心岩藻糖基化聚糖减少。PCa PSA 亚基中的这些 N-聚糖变化突出了糖基化作为 PCa 疾病标志物的重要性。
