Barrabés Sílvia, Farina-Gomez Noemi, Llop Esther, Puerta Angel, Diez-Masa Jose Carlos, Perry Antoinette, de Llorens Rafael, de Frutos Mercedes, Peracaula Rosa
Biology Department, Faculty of Science, University of Girona, Girona, Spain.
Department of Instrumental Analysis and Environmental Chemistry, Institute of Organic Chemistry, Spanish Research Council (IQOG-CSIC), Madrid, Spain.
Electrophoresis. 2017 Feb;38(3-4):408-416. doi: 10.1002/elps.201600432. Epub 2016 Oct 24.
Serum levels of Prostate-Specific Antigen (PSA) are not fully specific for prostate cancer (PCa) diagnosis and several efforts are focused on searching to improve PCa markers through the study of PSA subforms that could be cancer associated. We have previously reported by 2DE a decrease in the sialic acid content of PSA from PCa compared to benign prostatic hyperplasia patients based on the different proportion of the PSA spots. However, faster and more quantitative techniques, easier to automate than 2DE, are desirable. In this study, we examined the potential of CE for resolving PSA subforms in different samples and compared the results with those obtained by 2DE. We first fractionated by OFFGEL the subforms of PSA from seminal plasma according to their pIs and analyzed each separated fraction by 2DE and CE. We also analyzed PSA and high pI PSA, both from seminal plasma, and PSA from urine of a PCa patient. These samples with different PSA spots proportions by 2DE, due to different posttranslational modifications, also presented different CE profiles. This study shows that CE is a useful and complementary technique to 2DE for analyzing samples with different PSA subforms, which is of high clinical interest.
血清前列腺特异性抗原（PSA）水平对于前列腺癌（PCa）诊断并非完全特异，目前有多项研究致力于通过研究可能与癌症相关的PSA亚型来寻找更好的PCa标志物。我们之前通过二维电泳（2DE）报道，基于PSA斑点的不同比例，与良性前列腺增生患者相比，PCa患者PSA的唾液酸含量降低。然而，需要比2DE更快、更定量且更易于自动化的技术。在本研究中，我们研究了毛细管电泳（CE）解析不同样品中PSA亚型的潜力，并将结果与2DE所得结果进行比较。我们首先根据等电点通过等胶分离（OFFGEL）从精浆中分离PSA亚型，然后通过2DE和CE分析每个分离的组分。我们还分析了来自精浆的PSA和高pI PSA，以及一名PCa患者尿液中的PSA。由于不同的翻译后修饰，这些样品在2DE上具有不同的PSA斑点比例，其CE图谱也不同。本研究表明，对于分析具有不同PSA亚型的样品，CE是一种对2DE有用的补充技术，具有很高的临床价值。
