UMR CNRS 7213, Laboratoire de Biophotonique et Pharmacologie, Université de Strasbourg, Faculté de Pharmacie, 67401 Illkirch, France.
J Ethnopharmacol. 2010 Oct 28;132(1):246-50. doi: 10.1016/j.jep.2010.08.031. Epub 2010 Aug 18.
Parkia biglobosa leaves are traditionally used as an antihypertensive agent in Benin. The present study assessed the vasorelaxant activity of different Parkia biglobosa leaf extracts using isolated porcine coronary artery rings.
A hydroalcoholic leaf extract was submitted to a multi-step liquid-liquid fractionation with solvents of increasing polarity and the polyphenolic content of the different fractions was analyzed. Vascular reactivity of the different extracts was assessed using porcine coronary artery rings, in the presence or absence of specific pharmacological inhibitors.
The hydroalcoholic, ethyl acetate and butanolic extracts contained mainly procyanidins and monomeric flavonoids. Parkia biglobosa leaf crude extract induced a redox-sensitive endothelium-dependent relaxation mediated by both nitric oxide (NO) and endothelium-derived hyperpolarizing factor (EDHF). The fractionation of the butanolic extract generated 6 fractions, two of which induced stronger vasorelaxation than the original extract and they had a higher phenolic content.
Parkia biglobosa leaf extract is able to induce endothelium-dependent NO- and EDHF-mediated relaxation in porcine coronary artery rings. The vasorelaxant activity is dependent on their phenolic content and appears to involve mainly procyanidins.
在贝宁，大托叶云实的叶子传统上被用作降压药。本研究使用离体猪冠状动脉环评估了大托叶云实不同叶提取物的血管舒张活性。
将水醇提物进行多步液-液分馏，使用极性逐渐增加的溶剂，分析不同馏分的多酚含量。在存在或不存在特定的药理学抑制剂的情况下，使用猪冠状动脉环评估不同提取物的血管反应性。
水醇提物、乙酸乙酯提物和正丁醇提物主要含有原花青素和单体类黄酮。大托叶云实叶粗提取物诱导由一氧化氮（NO）和内皮衍生超极化因子（EDHF）介导的氧化还原敏感的内皮依赖性舒张。正丁醇提取物的分级产生了 6 个馏分，其中两个馏分比原始提取物引起更强的血管舒张作用，并且它们具有更高的酚类含量。
大托叶云实叶提取物能够诱导猪冠状动脉环中的内皮依赖性 NO 和 EDHF 介导的舒张。血管舒张活性取决于其酚类含量，似乎主要涉及原花青素。
