Ouédraogo Sylvin, Somé Noya, Ouattara Sounkalo, Kini Félix B, Traore Aristide, Bucher Bernard, Guissou I Pierre
Institut de Recherche en Sciences de la Santé 01 BP 7192 Ouagadougou01 Burkina Faso.
Afr J Tradit Complement Altern Med. 2011 Dec 29;9(2):260-5. doi: 10.4314/ajtcam.v9i2.12. eCollection 2012.
The authors report here the results of study on Parkia biglobosa seeds used in Burkina Faso for arterial hypertension treatment. Investigations were done on acute toxicity and vascular properties of fermented and roasted seeds. Acute toxicity test using mice, revealed by the intraperitoneal route a lethal dose 50 (LD50) of 1800 mg/kg and 1600 mg/kg of body weight for aqueous extract from roasted and fermented seeds respectively. According to the scale of Hodge and Sterner and that of the World Health Organization, such drugs would be classified lightly toxic. Oral administration (up to 3000 mg/kg) did not induce any death of animal. For the vascular properties, the effects of these products were tested on the aorta isolated from rats. The cumulative administration of extract from roasted and fermented seeds (0.1-10 mg/mL) in an organ bath induced a concentration-dependent relaxation of the aorta pre contracted by phenylephrine, with or without functional endothelium. The extracts (10 mg/mL) inhibited for 100% the contraction induced by phenylephrine. The EC50 values in presence and absence of endothelium were respectively of 5.37 ± 0.12 and 4.19 ± 1.02 mg/mL for fermented seeds; for roasted seeds these values were respectively, 5.39 ± 1.12 and 5.93 ± 0.95 mg/mL. Nevertheless, low concentration of roasted seeds (1-4 mg/mL) induced endothelium-dependent relaxation and this effect was inhibited by indomethacin (10⁻⁵M), and not by L-NAME (310⁻⁴M). These experimental results revealed a vasorelaxant effect of P. biglobosa seeds. P. biglobosa seems to act directly on the smooth muscle and via endothelium involving the generation of vasodilatating prostaglandins. This vasodilator effect would be in favor of an anti hypertensive property of P. biglobosa seeds.
作者在此报告了对布基纳法索用于治疗动脉高血压的大叶球花豆种子的研究结果。对发酵和烘焙种子的急性毒性和血管特性进行了研究。使用小鼠进行的急性毒性试验表明，经腹腔途径，烘焙种子和发酵种子的水提取物的半数致死量（LD50）分别为1800毫克/千克和1600毫克/千克体重。根据霍奇和斯特纳的标准以及世界卫生组织的标准，此类药物将被归类为轻度毒性。口服给药（高达3000毫克/千克）未导致任何动物死亡。对于血管特性，在从大鼠分离的主动脉上测试了这些产品的效果。在器官浴中累积给予烘焙和发酵种子的提取物（0.1 - 10毫克/毫升）可诱导由去氧肾上腺素预收缩的主动脉出现浓度依赖性舒张，无论有无功能性内皮。提取物（10毫克/毫升）可100%抑制去氧肾上腺素诱导的收缩。有内皮和无内皮时发酵种子的半数有效浓度（EC50）值分别为5.37±0.12和4.19±1.02毫克/毫升；对于烘焙种子，这些值分别为5.39±1.12和5.93±0.95毫克/毫升。然而，低浓度的烘焙种子（1 - 4毫克/毫升）诱导内皮依赖性舒张，且这种作用被吲哚美辛（10⁻⁵摩尔）抑制，而不被L - 精氨酸甲酯（3×10⁻⁴摩尔）抑制。这些实验结果揭示了大叶球花豆种子的血管舒张作用。大叶球花豆似乎直接作用于平滑肌，并通过内皮发挥作用，涉及血管舒张前列腺素的生成。这种血管舒张作用有利于大叶球花豆种子的抗高血压特性。
