Pedro Kourí Tropical Medicine Institute (IPK), PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Havana City, Cuba.
Diagn Microbiol Infect Dis. 2010 Sep;68(1):46-9. doi: 10.1016/j.diagmicrobio.2010.04.006.
In this work, the presence of NS1 protein as a possible early marker of dengue infection was studied in serum samples from confirmed adult patients with a primary and secondary dengue 4 infection. A total of 209 serum samples collected from day 2 up to day 7 of fever onset from 71 patients were tested by Platelia NS1 antigen capture ELISA kit (BioRad, Marnes-la-Coquette, France), and the results were compared with those obtained by capture antidengue virus IgM (MAC)-ELISA and ELISA inhibition method tests. The 83.3% of primary cases and 96.4% of secondary cases were NS1 positive. The kinetics of NS1 protein showed the highest values in optical density mean ratio or in percentage of positives between days 2 and 4. The results obtained in this study show the utility of the NS1 protein as a virologic early marker of dengue infection. Prospective studies should be carried out to confirm its utility as a prognostic marker of severe illness.
在这项工作中，研究了 NS1 蛋白作为登革热感染的早期标志物在确诊的成人原发性和复发性登革热 4 型感染患者血清样本中的存在情况。共检测了 71 例患者发热第 2 天至第 7 天采集的 209 份血清样本，采用 Platelia NS1 抗原捕获 ELISA 试剂盒（BioRad，Marnes-la-Coquette，法国），并将结果与捕获抗登革热病毒 IgM（MAC）-ELISA 和 ELISA 抑制法检测结果进行比较。83.3%的原发性病例和 96.4%的继发性病例 NS1 阳性。NS1 蛋白的动力学研究显示，在第 2 天至第 4 天之间，光密度平均比值或阳性百分比的数值最高。本研究的结果表明，NS1 蛋白可用作登革热感染的病毒学早期标志物。应开展前瞻性研究以确认其作为重症疾病预后标志物的效用。
