Department of Biochemistry 280, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
Biomaterials. 2010 Nov;31(32):8299-312. doi: 10.1016/j.biomaterials.2010.07.055. Epub 2010 Aug 19.
The in vivo performance of tissue-engineered constructs is often based on generally accepted read-out parameters, like (immuno)histology. In this study, high-density gene expression microarrays and gene ontology (GO) analysis were used as a read-out tool to identify the biological processes occurring after implantation of an acellular collagen-based skin construct using a rat full-thickness wound model. A freely-available program (DAVID) was used to identify up/downregulated biological processes (GO-terms) and results were compared to wound healing/regeneration without a construct. The entire process from RNA isolation to biological interpretation is explained step-by-step. Conventional (immuno)histology was used to validate the biological processes identified and indicate that microarray analysis may provide a valuable, fast and unbiased tool to evaluate the in vivo performance of tissue-engineered constructs. However, challenges remain e.g. with regards to the development of specific GO-terms and annotation of the (rat) genome.
体内组织工程构建体的性能通常基于（免疫）组织化学等公认的读出参数。在这项研究中，使用高密度基因表达微阵列和基因本体论 (GO) 分析作为读出工具，以确定在使用大鼠全层创面模型植入去细胞胶原基皮肤构建体后发生的生物学过程。使用一个免费程序 (DAVID) 来识别上调/下调的生物学过程 (GO 术语)，并将结果与没有构建体的创面愈合/再生进行比较。从 RNA 分离到生物学解释的整个过程都被逐步解释。常规（免疫）组织化学用于验证鉴定的生物学过程，并表明微阵列分析可能为评估组织工程构建体的体内性能提供有价值、快速和无偏的工具。然而，仍然存在挑战，例如关于特定 GO 术语的开发和（大鼠）基因组的注释。
