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Sexual reproduction is crucial for increasing the genetic diversity of populations and providing overwintering structures, such as perithecia and associated tissue, in the destructive plant pathogenic fungus . While mating-type genes serve as master regulators in fungal sexual reproduction, the molecular mechanisms underlying this process remain elusive. Winged-helix DNA-binding proteins are key regulators of embryogenesis and cell differentiation in higher eukaryotes. These proteins are implicated in the morphogenesis and development of several fungal species. However, their involvement in sexual reproduction remains largely unexplored in . Here, we investigated the function of winged-helix DNA-binding proteins in vegetative growth, conidiation, and sexual reproduction, with a specific focus on the , which is highly conserved among species. Deletion of resulted in an abnormal pattern characterized by a gradual increase in the expression of mating-type genes during sexual development, indicating its crucial role in the stage-specific genetic regulation of genes in the late stages of sexual development. Furthermore, using chromatin immunoprecipitation followed by sequencing analysis, we identified as a downstream gene of Fgwing27, which is essential for sexual reproduction. These findings underscore the significance of winged-helix DNA-binding proteins in fungal development and reproduction in , and highlight the pivotal role of Fgwing27 as a core genetic factor in the intricate genetic regulatory network governing sexual reproduction.IMPORTANCE is a devastating plant pathogenic fungus causing significant economic losses due to reduced crop yields. In Fusarium Head Blight epidemics, spores produced through sexual and asexual reproduction serve as inoculum, making it essential to understand the fungal reproduction process. Here, we focus on winged-helix DNA-binding proteins, which have been reported to play crucial roles in cell cycle regulation and differentiation, and address their requirement in the sexual reproduction of . Furthermore, we identified a highly conserved protein in as a key factor in self-fertility, along with the discovery of its direct downstream genes. This provides crucial information for constructing the complex genetic regulatory network of sexual reproduction and significantly contribute to further research on sexual reproduction in species.

性繁殖对于增加种群的遗传多样性和提供越冬结构（如子囊壳和相关组织）至关重要，而这些结构是破坏性植物病原真菌的特征。虽然交配型基因是真菌有性繁殖的主要调控因子，但这一过程的分子机制仍不清楚。翼状螺旋 DNA 结合蛋白是高等真核生物胚胎发生和细胞分化的关键调控因子。这些蛋白参与了几种真菌物种的形态发生和发育。然而，它们在真菌有性生殖中的作用在真菌中仍在很大程度上尚未被探索。在这里，我们研究了翼状螺旋 DNA 结合蛋白在营养生长、产孢和有性生殖中的功能，特别关注高度保守的交配型基因在晚性发育阶段的特异性遗传调控中的作用。此外，我们使用染色质免疫沉淀测序分析，鉴定了 Fgwing27 的下游基因，Fgwing27 是性繁殖所必需的。这些发现强调了翼状螺旋 DNA 结合蛋白在真菌发育和繁殖中的重要性，突出了 Fgwing27 作为核心遗传因素在调控性繁殖的复杂遗传调控网络中的关键作用。

重要性是一种破坏性的植物病原真菌，由于作物产量下降，造成了重大的经济损失。在镰刀菌穗枯病流行期间，通过有性和无性繁殖产生的孢子作为接种体，因此了解真菌的繁殖过程至关重要。在这里，我们重点研究了翼状螺旋 DNA 结合蛋白，这些蛋白已被报道在细胞周期调控和分化中发挥关键作用，并探讨了它们在的有性繁殖中的需求。此外，我们确定了中的一个高度保守的蛋白是自育性的关键因素，同时发现了其直接下游基因。这为构建复杂的性繁殖遗传调控网络提供了重要信息，并为进一步研究真菌物种的性繁殖做出了重要贡献。