Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo, 108-8477, Japan.
Fish Shellfish Immunol. 2011 Jan;30(1):425-9. doi: 10.1016/j.fsi.2010.08.005. Epub 2010 Aug 20.
Toll-like receptors (TLRs) are essential for activation of the innate immune system in response to invading pathogens. TLR14, which is unique to fish, has been identified in several fish species, but its function is unclear. In this study, Japanese flounder (Paralichthys olivaceus) TLR14 gene (JfTLR14) was cloned and its expression profiles were analyzed after infection with viral hemorrhagic septicemia virus, gram-positive Streptococcus iniae and gram-negative Edwardsiella tarda. The coding region of JfTLR14 cDNA was 2,607 bp, encoding 878 amino acid residues. JfTLR14 was highly expressed in head kidney of healthy flounder. In response to infection with VHSV and S. iniae, the JfTLR14 gene was up-regulated at only 1 day post-infection (dpi). However, E. tarda infection increased JfTLR14 gene expression from 1 to 6 dpi. These results imply that JfTLR14 participates more in the immune response against E. tarda infection than in the immune responses to other pathogen infections.
Toll 样受体 (TLRs) 是固有免疫系统对入侵病原体产生应答的关键。TLR14 是鱼类所特有的一种受体，已在多种鱼类中被鉴定出来，但它的功能尚不清楚。本研究克隆了牙鲆 TLR14 基因 (JfTLR14)，并分析了其在感染病毒性出血性败血症病毒、革兰阳性鳗弧菌和革兰阴性迟缓爱德华菌后的表达谱。JfTLR14 cDNA 的编码区为 2607 bp，编码 878 个氨基酸残基。健康牙鲆的头肾中 JfTLR14 表达量最高。在感染 VHSV 和 S. iniae 后，JfTLR14 基因仅在感染后 1 天被上调。然而，E. tarda 感染从 1 天至 6 天增加了 JfTLR14 基因的表达。这些结果表明，JfTLR14 更多地参与了对 E. tarda 感染的免疫反应，而不是对其他病原体感染的免疫反应。
