NanoScience Technology Center, Department of Mechanical, Materials, and Aerospace Engineering, School of Electrical Engineering and Computer Science, University of Central Florida, Orlando, FL 32826, United States.
Biosens Bioelectron. 2010 Oct 15;26(2):437-43. doi: 10.1016/j.bios.2010.07.097. Epub 2010 Aug 3.
This paper describes a novel thermal biosensing technique for the highly sensitive and selective detection of thrombin using RNA aptamer-functionalized phase change nanoparticles as thermal probes. The presence of thrombin in solution leads to attachment of nanoparticles onto a substrate modified with the same aptamer by forming sandwiched complexes. The phase changes of nanoparticles from solid to liquid adsorb heat energy and generate sharp melting peaks during linear temperature scans, where the positions and areas of the melting peaks reflect the presence and the amount of thrombin, respectively. A detection sensitivity of 22 nM is achieved on flat aluminum surfaces, and the sensitivity can be enhanced by four times using silicon nanopillar substrates that have higher surface area. The thermal detection is immune to colored species in solution and has been used directly to detect thrombin in serum samples. By combining the high specificity of aptamers and the large surface area of silicon nanostructures, the thermal signals obtained during phase change of nanoparticles provide a highly sensitive, selective and low-cost method for thrombin detection.
本文描述了一种新颖的热生物传感技术，用于使用 RNA 适体功能化的相变纳米粒子作为热探针，对凝血酶进行高灵敏度和选择性检测。溶液中的凝血酶存在会导致纳米粒子通过与相同适体形成夹心复合物附着在经相同适体修饰的基底上。纳米粒子从固态变为液态的相变会在线性温度扫描过程中吸收热能并产生尖锐的熔融峰，其中熔融峰的位置和面积分别反映了凝血酶的存在和数量。在平铝板表面上实现了 22 nM 的检测灵敏度，并且使用具有更高表面积的硅纳米柱基底可以将灵敏度提高四倍。热检测对溶液中的有色物质具有抗干扰性，并已直接用于检测血清样品中的凝血酶。通过结合适体的高特异性和硅纳米结构的大表面积，纳米粒子相变过程中的热信号提供了一种用于凝血酶检测的高灵敏度、选择性和低成本方法。
