University of Antwerp, Department of Biology, Laboratory of Ecophysiology, Biochemistry and Toxicology, Groenenborgerlaan 171, 2020 Antwerp, Belgium.
Biosens Bioelectron. 2010 Dec 15;26(4):1178-94. doi: 10.1016/j.bios.2010.07.033. Epub 2010 Jul 17.
A sensitive monitoring of contaminants in food and environment, such as chemical compounds, toxins and pathogens, is essential to assess and avoid risks for both, human and environmental health. To accomplish this, there is a high need for sensitive, robust and cost-effective biosensors that make real time and in situ monitoring possible. Due to their high sensitivity, selectivity and versatility, affinity-based biosensors are interesting for monitoring contaminants in food and environment. Antibodies have long been the most popular affinity-based recognition elements, however recently a lot of research effort has been dedicated to the development of novel recognition elements with improved characteristics, like specificity, stability and cost-efficiency. This review discusses three of these innovative affinity-based recognition elements, namely, phages, nucleic acids and molecular imprinted polymers and gives an overview of biosensors for food and environmental applications where these novel affinity-based recognition elements are applied.
对食品和环境中的污染物(如化学化合物、毒素和病原体)进行敏感监测,对于评估和避免人类健康和环境健康风险至关重要。为了实现这一目标,需要灵敏、稳健且具有成本效益的生物传感器,以便实现实时和现场监测。由于其高灵敏度、选择性和多功能性,基于亲和力的生物传感器在监测食品和环境中的污染物方面具有很大的吸引力。抗体一直是最受欢迎的基于亲和力的识别元件,但最近,人们投入了大量的研究精力来开发具有改进特性(如特异性、稳定性和成本效益)的新型识别元件。本文综述了这三种创新的基于亲和力的识别元件,即噬菌体、核酸和分子印迹聚合物,并概述了这些新型基于亲和力的识别元件在食品和环境应用中所应用的生物传感器。