Electronic and Computer Engineering Department, University of Limerick, Limerick, Ireland.
Biotechnol Adv. 2010 Mar-Apr;28(2):232-54. doi: 10.1016/j.biotechadv.2009.12.004. Epub 2009 Dec 16.
Food safety is a global health goal and the foodborne diseases take a major crisis on health. Therefore, detection of microbial pathogens in food is the solution to the prevention and recognition of problems related to health and safety. For this reason, a comprehensive literature survey has been carried out aiming to give an overview in the field of foodborne pathogen detection. Conventional and standard bacterial detection methods such as culture and colony counting methods, immunology-based methods and polymerase chain reaction based methods, may take up to several hours or even a few days to yield an answer. Obviously this is inadequate, and recently many researchers are focusing towards the progress of rapid methods. Although new technologies like biosensors show potential approaches, further research and development is essential before biosensors become a real and reliable choice. New bio-molecular techniques for food pathogen detection are being developed to improve the biosensor characteristics such as sensitivity and selectivity, also which is rapid, reliable, effective and suitable for in situ analysis. This paper not only offers an overview in the area of microbial pathogen detection but it also describes the conventional methods, analytical techniques and recent developments in food pathogen detection, identification and quantification, with an emphasis on biosensors.
食品安全是全球健康目标，食源性疾病对健康构成重大危机。因此，检测食品中的微生物病原体是预防和识别与健康和安全相关问题的解决方案。出于这个原因，我们进行了全面的文献调查，旨在概述食品病原体检测领域。传统和标准的细菌检测方法，如培养和菌落计数方法、基于免疫学的方法和基于聚合酶链反应的方法，可能需要数小时甚至数天才能得出答案。显然，这是不够的，最近许多研究人员都在关注快速方法的进展。尽管像生物传感器这样的新技术显示出了潜在的方法，但在生物传感器成为真正可靠的选择之前，还需要进一步的研究和开发。新的生物分子技术正在被开发用于提高生物传感器的特性，如灵敏度和选择性，也正在变得快速、可靠、有效和适合现场分析。本文不仅概述了微生物病原体检测领域，还描述了传统方法、分析技术以及食品病原体检测、鉴定和定量的最新进展，重点介绍了生物传感器。
