Faculty of Civil Engineering, Environmental Engineering Department, Istanbul Technical University, Istanbul, Turkey.
Bioresour Technol. 2013 Sep;144:410-9. doi: 10.1016/j.biortech.2013.06.121. Epub 2013 Jul 5.
The study evaluated acute impact of erythromycin and tetracycline on nitrification and organic carbon removal kinetics in mixed microbial culture. Acclimated biomass was obtained from a fill and draw reactor fed with peptone mixture selected as synthetic substrate and operated at a sludge age of 10 days. Acute inhibition was tested in batch reactors involving a control unit started solely with substrate and the others with additional doses of each antibiotic. Modeling indicated that both steps of nitrification were totally blocked by erythromycin. Tetracycline inhibited and retarded nitrification kinetics at 50 mg/L and stopped nitrite oxidation at 200 mg/L, leading to nitrite accumulation. Both antibiotics also affected organic carbon removal by inducing partial inactivation of the heterotrophic community in the culture, increased substrate storage and accelerated endogenous respiration, with a relatively slight impact on heterotrophic growth. Major inhibitory effect was on process stoichiometry, leading to partial utilization of organic substrate.
该研究评估了红霉素和四环素对混合微生物培养物硝化和有机碳去除动力学的急性影响。驯化生物量来自填充和抽取反应器，该反应器以作为合成底物的蛋白胨混合物为食，并以污泥龄为 10 天运行。在批处理反应器中进行急性抑制测试，其中一个控制单元仅用底物启动，而其他单元则添加了每种抗生素的额外剂量。模型表明，红霉素完全阻断了硝化的两个步骤。四环素在 50mg/L 时抑制和延迟硝化动力学，并在 200mg/L 时停止亚硝酸盐氧化，导致亚硝酸盐积累。两种抗生素还通过诱导培养物中异养群落的部分失活、增加底物储存和加速内源呼吸来影响有机碳去除，对异养生长的影响相对较小。主要的抑制作用是对工艺化学计量的影响，导致有机底物的部分利用。
