Graduate Institute of Applied Science of Living, Tainan University of Technology, Tainan, Taiwan.
Oral Oncol. 2010 Oct;46(10):767-72. doi: 10.1016/j.oraloncology.2010.07.011. Epub 2010 Aug 21.
Inflammation can be induced by cytokines, chemokines, and their receptors, and it is believed to be a risk factor on tumor initiation and progression. The contribution of CC chemokine ligand 5 (CCL5) and CC chemokine receptor 5 (CCR5) on the risk and prognosis of oral cancer is still poorly investigated. The aims of this study were to investigate the impacts of single nucleotide polymorphisms (SNPs) in CCL5 and CCR5 genes and the synergistic effects of these SNPs on the risk and clinicopathological characteristics of oral cancer. In this case-control study, a total of 253 oral cancer patients and 347 controls were recruited. The genetic polymorphisms of CCL5-28, -403 and CCR5-59029 were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) genotyping analysis. The results of statistical analysis showed that the subjects with CCL5-28 CG, CCL5-28 CG or GG, and CCL5-403 TT polymorphic genotype as well as the subjects with the combinations of CCL5-28 CG/-403 CT and CCL5-28 CG/-403 TT genotypes having a significant higher risk to oral cancer than those with wild-type genotypes. Moreover, the oral cancer patients with the combination of CCL5-28 CG/-403 TT genotype presented a lower risk for developing a moderately or poorly differentiated status as compared to those with the combination of CCL5-28 CC/-403 CC genotype. These results suggest that the SNPs in CCL5-28 and -403 genes could increase the risk to have oral cancer, and the combinative effect of CCL5-28 CG and -403 TT genes might also increase the oral cancer risk but reduce the clinicopathological development of oral cancer patients.
炎症可以由细胞因子、趋化因子及其受体诱导，并且被认为是肿瘤发生和进展的一个风险因素。CC 趋化因子配体 5（CCL5）和 CC 趋化因子受体 5（CCR5）在口腔癌风险和预后中的作用仍未得到充分研究。本研究旨在探讨 CCL5 和 CCR5 基因单核苷酸多态性（SNPs）及其对口腔癌风险和临床病理特征的协同作用。在这项病例对照研究中，共招募了 253 名口腔癌患者和 347 名对照。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）基因分型分析，对 CCL5-28、-403 和 CCR5-59029 的遗传多态性进行分析。统计分析结果表明，CCL5-28 CG、CCL5-28 CG 或 GG 以及 CCL5-403 TT 多态基因型的个体，以及 CCL5-28 CG/-403 CT 和 CCL5-28 CG/-403 TT 基因型组合的个体，患口腔癌的风险明显高于野生型基因型的个体。此外，与 CCL5-28 CC/-403 CC 基因型组合相比，CCL5-28 CG/-403 TT 基因型组合的口腔癌患者发生中、低分化状态的风险较低。这些结果表明，CCL5-28 和-403 基因中的 SNPs 可能增加患口腔癌的风险，并且 CCL5-28 CG 和-403 TT 基因的组合效应也可能增加口腔癌的风险，但降低口腔癌患者的临床病理发展。
