Christensen H, Bojesen A M, Bisgaard M
Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, 4 Stigbøjlen, DK-1870 Frederiksberg C, Denmark.
Int J Syst Evol Microbiol. 2011 Jul;61(Pt 7):1699-1704. doi: 10.1099/ijs.0.026518-0. Epub 2010 Aug 20.
Strains T138021-75(T), Pg19 and Pg20 (taxon 25 of Bisgaard) were isolated from guinea pigs and characterized. Strains T138021-75(T) and Pg20 showed identical 16S rRNA gene sequences and were distantly related to the published strain P224 with the highest 16S rRNA similarity of 98.6 %. These two strains showed 97.8 % sequence similarity with the type strain and other strains of Mannheimia glucosida and 97.3 % similarity with the type strain of Mannheimia varigena, but <97 % similarity with all other type strains of the genus Mannheimia, including Mannheimia haemolytica (96.9 %). Phylogenetic analysis of rpoB gene sequences showed that strain P224 had a distant position (89.9 % gene sequence similarity) compared with the three other strains (T138021-75(T), Pg20 and Pg19), which had identical gene sequences. These three novel strains also shared identical recN gene sequences. Phylogenetic analysis of the recN gene sequences showed a close relationship between the three novel strains and strain P224. The DNA-DNA reassociation value between strain T138021-75(T) and P224 was 81.6 % and 40.3 % between strain T138021-75(T) and the type strain of M. glucosida. Based on the DNA-DNA reassociation data, strain T138021-75(T) belonged to a separate species that was closely related to strain P224. Strain P224 differed from strains T138021-75(T), Pg20 and Pg19 in the following phenotypic characteristics: activity of ornithine carboxylase, hydrolysis of glycosides, and acid formation from maltose, dextrin, melibiose and raffinose, as well as reactions for α-galactosidase and β-xylosidase. Whole genome similarity calculations based on recN gene sequences showed that strains T138021-75(T) and P224 were related at the species level (0.932), whereas 16S rRNA and partial rpoB gene sequence comparisons showed a more divergent position of strain P224 compared with the novel strains, including a different host of isolation. The results showed that the three strains of taxon 25 represent a novel species for which the name Mannheimia caviae sp. nov. is proposed. The type strain, T138021-75(T) ( = CCUG 59995(T) = DSM 23207(T)) was isolated from purulent conjunctivitis in guinea pigs. Previous publications have documented both ubiquinones and demethylmenaquinone to be present in the type strain. The G+C content of the DNA of the type strain has been found to be 41.4 mol% (T(m)).
从豚鼠中分离出菌株T138021 - 75(T)、Pg19和Pg20（Bisgaard分类单元25）并进行了特性鉴定。菌株T138021 - 75(T)和Pg20显示出相同的16S rRNA基因序列，与已发表的菌株P224亲缘关系较远，16S rRNA最高相似度为98.6%。这两个菌株与葡糖曼海姆菌的模式菌株及其他菌株的序列相似度为97.8%，与变异曼海姆菌的模式菌株相似度为97.3%，但与曼海姆菌属的所有其他模式菌株相似度均<97%，包括溶血曼海姆菌（96.9%）。rpoB基因序列的系统发育分析表明，菌株P224与其他三个菌株（T138021 - 75(T)、Pg20和Pg19）相比处于较远的位置（基因序列相似度为89.9%），后三个菌株具有相同的基因序列。这三个新菌株也共享相同的recN基因序列。recN基因序列的系统发育分析表明，这三个新菌株与菌株P224关系密切。菌株T138021 - 75(T)与P224之间的DNA - DNA重结合值为81.6%，菌株T138021 - 75(T)与葡糖曼海姆菌模式菌株之间为40.3%。基于DNA - DNA重结合数据，菌株T138021 - 75(T)属于一个与菌株P224密切相关的独立物种。菌株P224在以下表型特征上与菌株T138021 - 75(T)、Pg20和Pg19不同：鸟氨酸羧化酶活性、糖苷水解、麦芽糖、糊精、蜜二糖和棉子糖产酸，以及α - 半乳糖苷酶和β - 木糖苷酶反应。基于recN基因序列的全基因组相似度计算表明，菌株T138021 - 75(T)和P224在种水平上相关（0.932），而16S rRNA和部分rpoB基因序列比较显示，与新菌株相比，菌株P224的位置更为不同，包括不同的分离宿主。结果表明，分类单元25中的这三个菌株代表一个新物种，为此提出名称为豚鼠曼海姆菌（Mannheimia caviae sp. nov.）。模式菌株T138021 - 75(T)（=CCUG 59995(T)=DSM 23207(T)）从豚鼠的脓性结膜炎中分离得到。先前的出版物已证明模式菌株中存在泛醌和去甲基甲萘醌。已发现模式菌株DNA的G + C含量为41.4 mol%（T(m)）。
Zotero 插件