Kwack Kyu Bum, Song Hee Jin, Pyun Jung A, Lee Kwang Jae, Cho Sung Won
Department of Biomedical Science, College of Life Science, CHA University, Seongnam, Korea.
Korean J Gastroenterol. 2010 Aug;56(2):78-82. doi: 10.4166/kjg.2010.56.2.78.
BACKGROUND/AIMS: Oncogenic RAS gene mutations have been frequently observed in many tumor types, and their associations with various cancers were reported. This study was conducted to evaluate the association between H-RAS T81C polymorphism and gastric cancer development.
H-RAS T81C polymorphism was genotyped in 321 chronic gastritis (ChG) and 151 gastric cancer (GC) patients using GoldenGate Assay kit. Logistic regression analysis adjusted for age and gender was performed to identify the differences of genotype and allele distributions between the each group.
All ChG and GC patients were in Hardy-Weinberg equilibrium. When the frequencies of H-RAS T81C genotype in each group were compared, the homozygous type of major allele TT was more frequent in GC group (62.9%) than ChG group (57.3%), while the frequencies of heterozygous type TC and homozygous type of minor allele CC were higher in ChG group than GC group (39.3% vs. 33.8%, 3.4% vs. 3.3%, respectively). In the results of logistic regression analyses adjusted for age and gender, the odds ratios were 0.845 (0.604-1.182), 0.799 (0.556-1.147), 0.741 (0.493-1.114) and 1.094 (0.366-3.270) for allele, codominant, dominant and recessive models, respectively. However, significant difference was not observed between two groups in any models.
H-RAS T81C polymorphism was not associated with gastric cancer development in a Korean population.
背景/目的：致癌性RAS基因突变在多种肿瘤类型中经常被观察到，并且有报道称其与各种癌症有关。本研究旨在评估H-RAS T81C多态性与胃癌发生之间的关联。
使用GoldenGate检测试剂盒对321例慢性胃炎（ChG）患者和151例胃癌（GC）患者进行H-RAS T81C多态性基因分型。进行年龄和性别校正的逻辑回归分析，以确定每组之间基因型和等位基因分布的差异。
所有ChG和GC患者均处于Hardy-Weinberg平衡状态。比较每组中H-RAS T81C基因型的频率时，GC组中主要等位基因TT的纯合型（62.9%）比ChG组（57.3%）更常见，而ChG组中杂合型TC和次要等位基因CC的纯合型频率高于GC组（分别为39.3%对33.8%，3.4%对3.3%）。在年龄和性别校正的逻辑回归分析结果中，等位基因、共显性、显性和隐性模型的优势比分别为0.845（0.604 - 1.182）、0.799（0.556 - 1.147）、0.741（0.493 - 1.114）和1.094（0.366 - 3.270）。然而，在任何模型中两组之间均未观察到显著差异。
在韩国人群中，H-RAS T81C多态性与胃癌发生无关。
