Rostami Masoumeh, Kalaei Zahra, Pourhoseingholi Mohamad Amin, Kadivar Mehdi
Biochemistry department, Pasteur Institute of Iran, Tehran, Iran.
Department of Biostatistics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gastroenterol Hepatol Bed Bench. 2013 Summer;6(3):146-51.
The aim of this study was to investigate relation between H-ras T81C polymorphism and some of the important risk factors in gastric adenocarcinoma (GA).
GA is one of the leading causes of cancer death in most countries. RAS gene is an important member in the PI3K-AKT signaling and the single nucleotide polymorphism at H-rasc DNA position 81 has been demonstrated has an important role in tumor genesis.
In this study, we carried out single-nucleotide polymorphism analysis in an Iranian population. A total of 100 patients with gastric adenocarcinoma and 100 controls were examined for the presence of T81C H-ras polymorphism using PCR- RFLP assay.
Statistical analysis revealed no relationship significant between TT, TC, CC and risk of GA, but, there was a poorly relation between male patient with C-carrier genotype and increasing risk of GA (P=0.07). Also, we investigate effect of four important risk factors for GA. There was a statistically significant difference between increasing of age and susceptibility for GA (OR=1.106, 95%CI=1.073-1.139, P < 0.001). We observed a statistically significant between smoking and T81C polymorphism C-carrier genotypes (OR=3.98, 95%CI=1.831-8.68, P < 0.001) as this individual had three-time risk for GA. We did not show a significant association between three main genotypes and H. pylori infection for risk of GA.
These results suggested that there is no relationship between T81C-HRAS polymorphism and gastric cancer risk in Iranian patients. But, gender (male in our study) and the other risk factor described above have an important role in developing of GA.
本研究旨在探讨H-ras基因T81C多态性与胃腺癌（GA）一些重要危险因素之间的关系。
在大多数国家，GA是癌症死亡的主要原因之一。RAS基因是PI3K-AKT信号通路中的重要成员，已证明H-ras基因DNA位置81处的单核苷酸多态性在肿瘤发生中起重要作用。
在本研究中，我们对伊朗人群进行了单核苷酸多态性分析。使用PCR-RFLP分析法对100例胃腺癌患者和100例对照者进行T81C H-ras多态性检测。
统计分析显示，TT、TC、CC基因型与GA风险之间无显著相关性，但携带C等位基因的男性患者与GA风险增加之间存在较弱的相关性（P=0.07）。此外，我们研究了GA的四个重要危险因素的影响。年龄增长与GA易感性之间存在统计学显著差异（OR=1.106，95%CI=1.073-1.139，P<0.001）。我们观察到吸烟与T81C多态性C等位基因携带者基因型之间存在统计学显著相关性（OR=3.98，95%CI=1.831-8.68，P<0.001），因为这类个体患GA的风险是三倍。我们未发现三种主要基因型与幽门螺杆菌感染对GA风险的显著关联。
这些结果表明，在伊朗患者中，T81C-HRAS多态性与胃癌风险之间无相关性。但是，性别（我们研究中的男性）和上述其他危险因素在GA的发生发展中起重要作用。
