胃癌患者中连接蛋白37基因C1019T多态性与幽门螺杆菌感染的相关性
Association between C1019T polymorphism in the connexin 37 gene and Helicobacter pylori infection in patients with gastric cancer.
作者信息
Jing Yuan-Ming, Guo Su-Xia, Zhang Xiao-Ping, Sun Ai-Jing, Tao Feng, Qian Hai-Xin
机构信息
Department of Gastrointestinal Surgery, Affiliated People's Hospital of Zhejing University, Shaoxing, Zhejiang, China.
出版信息
Asian Pac J Cancer Prev. 2012;13(5):2363-7. doi: 10.7314/apjcp.2012.13.5.2363.
OBJECTIVE
To investigate the association between the connexin 37 C1019T polymorphism and Helicobacter pylori infection in patients with gastric cancer.
METHODS
388 patients with gastric cancer (GC), 204 with chronic superficial gastritis (CSG) were studied. H. pylori was detected by gastric mucosal biopsies biopsy dyeing method. Connexin 37 gene polymorphism 1019 site genotypes were determined by gene sequencing technology. Genotypes and alleles frequencies were compared.
RESULTS
(1) Connexin37 gene 1019 site distribution frequency (CC type, TC type, TT type) in the CSG group was 18.1%, 45.1% and 36.8%; in the stomach cancer group it was 35.1%, 45.9% and 19.%, conforming to the Hardy-Weinberg euilibrium. (2) In comparison with CSG group, the frequency of Connexin37 C allele was higher in the gastric cancer group (58.0% vs 40.7%, OR=2.01, 95%CI=1.58-2.57, P<0.01). The prevalence of gastric cancer risk was significantly increased in the carriers of C allele (CC+TC) than in TT homozygote (OR=2.47, 5%CI=1.68-3.610. (3) Gastric cancer patients complicated with Hp infection 211 cases, gastric cancer group of the male patients with HP positive patients with 187 cases, 40 cases of female patients with negative patients, 24 cases were HP positive, negative in 137 cases, control group male patients, 28 cases were Hp positive, negative in 95 patients, female patients with Hp positive 6 cases, 75 cases were negative. On hierarchical analysis, the male group OR value was 15.9 (95%CI to 9.22-27.3), and the female OR was 2.19 (95%CI 0.88-5.59), indicating a greater contribution in males (P<0.01). After elimination of gender effects, positive HP and gastric cancer were closely related (OR 8.82, 95% CI: 5.45-14.3). (4) The distribution frequency of C allele in patients with Hp infection was much higher than that in Hp negative cases in the GC group (64.5% vs 47.0%, OR=2.05, 95%CI=1.54-2.74, P<0.01). Compared with TT homozygotes, (CC+TC) genotype prevalence of gastric cancer risk increased significantly (OR=2.96, 5%CI=1.76-2.99).
CONCLUSION
The T allele in the connexin37 gene might not only be associated with gastric cancer but also with H. pylori infection.
目的
探讨胃癌患者中连接蛋白37（Connexin37）基因C1019T多态性与幽门螺杆菌（Helicobacter pylori）感染之间的关联。
方法
对388例胃癌患者（GC）和204例慢性浅表性胃炎（CSG）患者进行研究。采用胃黏膜活检染色法检测幽门螺杆菌。通过基因测序技术确定连接蛋白37基因多态性1019位点的基因型。比较基因型和等位基因频率。
结果
（1）CSG组中连接蛋白37基因1019位点的分布频率（CC型、TC型、TT型）分别为18.1%、45.1%和36.8%；胃癌组中分别为35.1%、45.9%和19.0%，符合Hardy-Weinberg平衡。（2）与CSG组相比，胃癌组中连接蛋白37基因C等位基因频率更高（58.0%对40.7%，OR = 2.01，95%CI = 1.58 - 2.57，P < 0.01）。C等位基因携带者（CC + TC）患胃癌的风险显著高于TT纯合子（OR = 2.47，5%CI = 1.68 - 3.61）。（3）胃癌合并Hp感染患者211例，胃癌组男性Hp阳性患者187例，女性患者40例，Hp阴性患者24例，阴性患者137例，对照组男性患者Hp阳性28例，阴性95例，女性患者Hp阳性6例，阴性75例。分层分析显示，男性组OR值为15.9（95%CI为9.22 - 27.3），女性组OR值为2.19（95%CI为0.88 - 5.59），表明男性的贡献更大（P < 0.01）。消除性别影响后，Hp阳性与胃癌密切相关（OR = 8.82，95%CI：5.45 - 14.3）。（4）GC组中Hp感染患者的C等位基因分布频率远高于Hp阴性患者（64.5%对47.0%，OR = 2.05，95%CI = 1.54 - 2.74，P < 0.01）。与TT纯合子相比，（CC + TC）基因型患胃癌的风险显著增加（OR = 2.96，5%CI = 1.76 - 2.99）。
结论
连接蛋白37基因中的T等位基因可能不仅与胃癌有关，还与幽门螺杆菌感染有关。
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