Life Sciences Institute and Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Autophagy. 2010 Oct;6(7):950-1. doi: 10.4161/auto.6.7.13009. Epub 2010 Oct 14.
In macroautophagy (hereafter autophagy), a morphological hallmark is the formation of double-membrane vesicles called autophagosomes that sequester and deliver cytoplasmic components to the lysosome/vacuole for degradation. This process begins with an initial sequestering compartment, the phagophore, which expands into the mature autophagosome. A tremendous amount of work has been carried out to elucidate the mechanism of how the autophagosome is formed. However, an important missing piece in this puzzle is where the membrane comes from. Independent lines of evidence have shown that preexisting organelles may continuously supply lipids to support autophagosome formation. In our analysis, we identified several components of the late stage secretory pathway that may redirect Golgi-derived membrane to autophagosome formation in response to starvation conditions.
在巨自噬（下文简称自噬）中，一个形态学标志是双层膜囊泡的形成，称为自噬体，它可以隔离和将细胞质成分输送到溶酶体/液泡进行降解。这个过程始于一个初始的隔离区，即吞噬体，它扩展成成熟的自噬体。已经进行了大量的工作来阐明自噬体形成的机制。然而，这个难题中一个重要的缺失部分是膜来自何处。独立的证据表明，预先存在的细胞器可能不断提供脂质来支持自噬体的形成。在我们的分析中，我们鉴定了晚期分泌途径的几个成分，这些成分可能在饥饿条件下将高尔基体衍生的膜重新定向到自噬体的形成。
