Martínez-Sobrido Luis, García-Sastre Adolfo
Department of Microbiology and Immunology, University of Rochester, USA.
J Vis Exp. 2010 Aug 3(42):2057. doi: 10.3791/2057.
Efforts by a number of influenza research groups have been pivotal in the development and improvement of influenza A virus reverse genetics. Originally established in 1999 plasmid-based reverse genetic techniques to generate recombinant viruses have revolutionized the influenza research field because specific questions have been answered by genetically engineered, infectious, recombinant influenza viruses. Such studies include virus replication, function of viral proteins, the contribution of specific mutations in viral proteins in viral replication and/or pathogenesis and, also, viral vectors using recombinant influenza viruses expressing foreign proteins.
多个流感研究小组的努力对甲型流感病毒反向遗传学的发展和改进起到了关键作用。1999年最初建立的基于质粒的反向遗传技术用于产生重组病毒，彻底改变了流感研究领域，因为通过基因工程改造的、具有感染性的重组流感病毒已经回答了一些特定问题。这些研究包括病毒复制、病毒蛋白的功能、病毒蛋白中的特定突变在病毒复制和/或发病机制中的作用，以及使用表达外源蛋白的重组流感病毒作为病毒载体。
