一种基于适体引发的滚环扩增的用于超灵敏检测蛋白质的简便扫描策略。
A facile scanometric strategy for ultrasensitive detection of protein using aptamer-initiated rolling circle amplification.
机构信息
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, P.R. China.
出版信息
Chem Commun (Camb). 2010 Sep 28;46(36):6720-2. doi: 10.1039/c002078h. Epub 2010 Aug 23.
DOI:10.1039/c002078h
PMID:20730209
Abstract
A pragmatic and simple strategy was developed for ultrasensitive detection of protein with good specificity and low matrix effect, which combined aptamer-initiated rolling circle amplification with a Au nanoparticle probe and convenient scanometric readout.
摘要
一种实用且简单的策略被开发出来，用于超灵敏检测蛋白质，具有良好的特异性和低基质效应，该策略结合了适体引发的滚环扩增与 Au 纳米颗粒探针以及便捷的扫描计读数。
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