基于斑点印迹法的适体-金纳米颗粒缀合物对蛋白质的超灵敏比色检测。
Ultrasensitive colorimetric detection of protein by aptamer-Au nanoparticles conjugates based on a dot-blot assay.
作者信息
Wang Yuling, Li Dan, Ren Wen, Liu Zuojia, Dong Shaojun, Wang Erkang
机构信息
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, and Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
出版信息
Chem Commun (Camb). 2008 Jun 14(22):2520-2. doi: 10.1039/b801055b. Epub 2008 Mar 31.
A simple, rapid and ultrasensitive colorimetric detection of protein using aptamer-Au nanoparticles (AuNPs) conjugates based on a dot-blot array has been developed, which was combined with the unique optical properties of AuNPs, enabling the visual detection of protein within minutes without any instrument.
基于斑点印迹阵列，利用适配体-金纳米颗粒（AuNPs）缀合物开发了一种简单、快速且超灵敏的蛋白质比色检测方法，该方法结合了AuNPs独特的光学性质，无需任何仪器即可在几分钟内实现蛋白质的可视化检测。
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