Christo S W, Absher T M, Boehs G
Departamento de Biologia Geral, Universidade Estadual de Ponta Grossa, Curitiba, PR, Brazil.
Braz J Biol. 2010 Aug;70(3):645-50. doi: 10.1590/s1519-69842010000300023.
In this study we describe the morphology of the larval shell of three oyster species of Crassostrea genus. Two species, C. rhizophorae and C. brasiliana, are native to the Brazilian coast, and C. gigas is an introduced species. Samples of laboratory reared larvae, obtained through artificial fertilisation, were collected at intervals during the cultivation process for analysis using Scanning Electron Microscopy (SEM). Prodissoconch morphology was observed in relation to the presence, position, form and number of teeth in the three larval stages: D-shaped larva, umbo larva and pediveliger. Characteristic of D-shaped larvae of C. rhizophorae was the total absence of teeth in the provinculum area while C. brasiliana and C. gigas had two anterior and two posterior teeth in each valve. In the umbo larval phase, the three species had the same number of teeth in each valve: two posterior and two anterior teeth in the right valve and three posterior and three anterior in the left valve. In the pediveliger stage the three species could be differentiated by the number of anterior teeth of the right valve: C. rhizophorae had two teeth, C. brasiliana one tooth and C. gigas three teeth.
在本研究中，我们描述了巨蛎属三种牡蛎幼虫壳的形态。其中两种，即巴西巨蛎和巴西巨牡蛎，原产于巴西海岸，而太平洋牡蛎是引进物种。通过人工授精获得的实验室饲养幼虫样本，在培养过程中间隔采集，用于扫描电子显微镜（SEM）分析。观察了三个幼虫阶段（D形幼虫、壳顶幼虫和面盘幼虫）原壳的形态，包括齿的存在、位置、形态和数量。巴西巨蛎D形幼虫的特征是原壳区域完全没有齿，而巴西巨牡蛎和太平洋牡蛎每个瓣膜有两颗前齿和两颗后齿。在壳顶幼虫阶段，三种牡蛎每个瓣膜的齿数相同：右瓣膜有两颗后齿和两颗前齿，左瓣膜有三颗后齿和三颗前齿。在面盘幼虫阶段，三种牡蛎可通过右瓣膜前齿的数量来区分：巴西巨蛎有两颗齿，巴西巨牡蛎有一颗齿，太平洋牡蛎有三颗齿。
