Wang Taoni, Quan Weiming, Cheng Qiqun, Fan RuiLang
Key Laboratory of Oceanic and Polar Fisheries, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
Mitochondrial DNA B Resour. 2018 Aug 27;3(2):892-895. doi: 10.1080/23802359.2018.1501299.
Oyster reefs have important ecological functions and environmental service values, such as filtering water, providing fish habitats, maintaining biodiversity, and carbon sequestration. Oyster reef recovery has become one of the most important means for fishery resource conservation and ecosystems restoration of coastal regions and are two important reef oysters along the coast of China. However, it is very difficult to identify the larvae of these two oysters. To solve this problem, we developed two pairs of novel species-specific PCR primers for identifying these two oysters. The forward -specific primer is Cs-F: 5'-CGAAGAGGGGCATGATAAATGAGG-3', and the reverse primer is Cs-R: 5'-ATATGAACTTCTCCAACCTCCCC-3', both of them within the scope of mitochondrial ND5 gene. The forward -specific primer is Ca-F: 5'-GGGCAAATAAAAGGCAAAACCC-3', located between tRNA and tRNA, and the reverse primer is Ca-R: 5'-CATAAACTTCTGCAAGACTCCC-3', lies between tRNA and small subunit ribosomal RNA (ssrRNA). These two primers can identify the larvae of and from other oysters rapidly and effectively, with extremely high accurate rate (=100%). This molecular identification method does not require prior sorting of larvae. These two specific primers are good contribution to molecular identification of , and other oysters, and could be a useful tool for oyster larvae identification and ecological restoration.
牡蛎礁具有重要的生态功能和环境服务价值，如过滤水质、提供鱼类栖息地、维持生物多样性以及碳固存。牡蛎礁恢复已成为沿海地区渔业资源保护和生态系统恢复的最重要手段之一，[此处原文缺失两种牡蛎的具体名称]是中国沿海两种重要的礁牡蛎。然而，很难识别这两种牡蛎的幼虫。为了解决这个问题，我们开发了两对新型物种特异性PCR引物来识别这两种牡蛎。正向特异性引物为Cs-F：5'-CGAAGAGGGGCATGATAAATGAGG-3'，反向引物为Cs-R：5'-ATATGAACTTCTCCAACCTCCCC-3'，它们都在线粒体ND5基因范围内。正向特异性引物为Ca-F：5'-GGGCAAATAAAAGGCAAAACCC-3'，位于tRNA和tRNA之间，反向引物为Ca-R：5'-CATAAACTTCTGCAAGACTCCC-3'，位于tRNA和小亚基核糖体RNA（ssrRNA）之间。这两种引物能够快速、有效地从其他牡蛎中识别出[此处原文缺失两种牡蛎的具体名称]的幼虫，准确率极高（=100%）。这种分子鉴定方法不需要事先对幼虫进行分类。这两种特异性引物对[此处原文缺失两种牡蛎的具体名称]及其他牡蛎的分子鉴定有很大贡献，并且可能成为牡蛎幼虫鉴定和生态恢复的有用工具。
