Department of General and Visceral Surgery, University of Freiburg, Hugstettter Strasse 55, Freiburg, Germany.
World J Gastroenterol. 2010 Aug 28;16(32):4031-8. doi: 10.3748/wjg.v16.i32.4031.
To assess the effects of bile and pancreatic juice on structural and mechanical resistance of extracellular matrices (ECMs) in vitro.
Small-intestinal submucosa (SIS), porcine dermal matrix (PDM), porcine pericardial matrix (PPM) and bovine pericardial matrix (BPM) were incubated in human bile and pancreatic juice in vitro. ECMs were examined by macroscopic observation, scanning electron microscopy (SEM) and testing of mechanical resistance.
PDM dissolved within 4 d after exposure to bile or pancreatic juice. SIS, PPM and PDM retained their integrity for > 60 d when incubated in either digestive juice. The effect of bile was found to be far more detrimental to mechanical stability than pancreatic juice in all tested materials. In SIS, the loss of mechanical stability after incubation in either of the digestive secretions was less distinct than in PPM and BPM [mFmax 4.01/14.27 N (SIS) vs 2.08/5.23 N (PPM) vs 1.48/7.89 N (BPM)]. In SIS, the extent of structural damage revealed by SEM was more evident in bile than in pancreatic juice. In PPM and BPM, structural damage was comparable in both media.
PDM is less suitable for support of gastrointestinal healing. Besides SIS, PPM and BPM should also be evaluated experimentally for gastrointestinal indications.
评估胆汁和胰液对细胞外基质（ECM）体外结构和机械阻力的影响。
将小肠黏膜下层（SIS）、猪真皮基质（PDM）、猪心包基质（PPM）和牛心包基质（BPM）在人胆汁和胰液中进行体外孵育。通过宏观观察、扫描电子显微镜（SEM）和机械阻力测试来检查 ECM。
PDM 在暴露于胆汁或胰液后 4 天内溶解。当在任何一种消化液中孵育时，SIS、PPM 和 PDM 均能保持完整性超过 60 天。与胰液相比，胆汁对所有测试材料的机械稳定性的破坏作用要大得多。在 SIS 中，在任何一种消化液中孵育后机械稳定性的丧失都不如 PPM 和 BPM 明显[mFmax 4.01/14.27 N（SIS）比 2.08/5.23 N（PPM）比 1.48/7.89 N（BPM）]。在 SIS 中，SEM 显示的结构损伤程度在胆汁中比在胰液中更为明显。在 PPM 和 BPM 中，两种介质中的结构损伤相当。
PDM 不太适合支持胃肠道愈合。除了 SIS 外，还应实验性地评估 PPM 和 BPM 是否适用于胃肠道适应证。
