Petrova E S, Chumasov E I
Arkh Anat Gistol Embriol. 1990 Oct;99(10):26-32.
By means of morphological methods dynamics of the spinal cord development in 14-day-old rat embryos implanted into the sciatic nerve of mature rats have been studied. The implants preserve their viability during 5 months after the operation and their cells continue to differentiate beginning from neuroepithelial cells and neuroblasts up to young and mature neurons with histotypical signs of motoneurons. In 6 h and 1 day after transplantation the neuroepithelial cells continue their mitotic division. In 3 days, however, their mitotic activity decreases essentially and differentiation of neuroblasts begins. In 7 days the implants consist mainly of differentiated neuroblasts and glial cells. As demonstrates electron microscopy, in 30 days after the operation in the implants there is a well developed neuropil, where mature neurons, myelinated axons are situated and synaptic contacts are present.
通过形态学方法，对植入成年大鼠坐骨神经的14日龄大鼠胚胎脊髓发育的动态过程进行了研究。植入物在手术后5个月内保持其活力，其细胞从神经上皮细胞和成神经细胞开始持续分化，直至具有运动神经元组织学特征的年轻和成熟神经元。移植后6小时和1天时，神经上皮细胞继续进行有丝分裂。然而，在3天时，它们的有丝分裂活性基本下降，成神经细胞开始分化。在7天时，植入物主要由分化的成神经细胞和神经胶质细胞组成。电子显微镜显示，手术后30天时，植入物中有发育良好的神经纤维网，其中有成熟神经元、有髓轴突，并且存在突触联系。
