Endo-Capron S, Renier A, Janson X, Kheuang L, Jaurand M C
INSERM-U139, Laboratoire de Toxicologie Cellulaire et Moléculaire de l'Environnement, CHU Henri Mondor, 94010 Créteil, France.
Toxicol In Vitro. 1993 Jan;7(1):7-14. doi: 10.1016/0887-2333(93)90107-g.
The genotoxicity of three samples of talc has been determined using in vitro cell systems previously developed for testing asbestos fibres. The talc samples used consisted of particles of respirable size in order to test the effect of particles likely to be deposited in the lung. Genotoxicity was tested in cultures of rat pleural mesothelial cells (RPMC) using genotoxicity assays for unscheduled DNA synthesis (UDS) and sister chromatid exchanges (SCEs). The effects were compared with those obtained with negative controls (attapulgite and anatase) and positive controls (chrysotile and crocidolite asbestos). In contrast to asbestos, none of the talc samples, nor the negative controls, induced enhancement of UDS or SCEs in treated cultures in comparison with the untreated cultures.
使用先前开发的用于测试石棉纤维的体外细胞系统，测定了三个滑石粉样品的遗传毒性。所使用的滑石粉样品由可吸入大小的颗粒组成，以便测试可能沉积在肺部的颗粒的影响。使用非程序性DNA合成（UDS）和姐妹染色单体交换（SCE）的遗传毒性测定法，在大鼠胸膜间皮细胞（RPMC）培养物中测试遗传毒性。将这些影响与阴性对照（凹凸棒石和锐钛矿）和阳性对照（温石棉和青石棉）所获得的影响进行比较。与石棉不同，与未处理的培养物相比，滑石粉样品和阴性对照均未在处理过的培养物中诱导UDS或SCE增强。
