细胞培养基紫外线照射对雏鸡胚胎肝细胞中多氯联苯介导的卟啉积累和EROD诱导的影响。

Effects of UV irradiation of cell culture medium on PCB-mediated porphyrin accumulation and EROD induction in chick embryo hepatocytes.

作者信息

Lorenzen A, James C A, Kennedy S W

机构信息

Environment Canada, Canadian Wildlife Service, National Wildlife Research Centre, Hull, Quebec, Canada K1A 0H3.

出版信息

Toxicol In Vitro. 1993 Mar;7(2):159-66. doi: 10.1016/0887-2333(93)90127-q.

DOI:10.1016/0887-2333(93)90127-q

PMID:20732185

Abstract

Primary cultures of chick embryo hepatocytes were maintained in Waymouth's MD 705/1 medium that was unexposed or had been exposed for 4 hr to 366 nm ultraviolet (UV) light. The cultures were assayed for 3,3',4,4'-tetrachlorobiphenyl (3,3',4,4'-TCB)-mediated porphyrin accumulation and ethoxyresorufin-O-deethylase (EROD) induction. A decrease in 3,3',4,4'-TCB-mediated porphyrin accumulation, due in part to tryptophan depletion after exposure of the cell culture medium to UV light, was observed. In contrast, EROD activity of cells maintained in irradiated medium was shown to increase over basal levels, possibly due to the presence of photoproducts of medium components. Further studies indicated that photoproducts obtained after irradiation of a concentrated aqueous solution of tryptophan were inducers of EROD activity in primary chick embryo hepatocytes.

摘要

鸡胚肝细胞原代培养物维持在未暴露或已暴露于366nm紫外线（UV）4小时的Waymouth's MD 705/1培养基中。对培养物进行3,3',4,4'-四氯联苯（3,3',4,4'-TCB）介导的卟啉积累和乙氧基异吩恶唑酮-O-脱乙基酶（EROD）诱导分析。观察到3,3',4,4'-TCB介导的卟啉积累减少，部分原因是细胞培养基暴露于紫外线后色氨酸耗竭。相比之下，维持在辐照培养基中的细胞的EROD活性显示超过基础水平增加，这可能是由于培养基成分的光产物的存在。进一步的研究表明，色氨酸浓水溶液辐照后获得的光产物是原代鸡胚肝细胞中EROD活性的诱导剂。