Department of Anesthesiology and Pain Medicine, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan.
Neuroscience. 2010 Nov 10;170(4):1028-34. doi: 10.1016/j.neuroscience.2010.08.014. Epub 2010 Aug 21.
Heat shock protein 27 (HSP27), a low-molecular-weight HSP, is recognized as a molecular chaperone. In response to various stimuli, HSP27 expression is induced in the CNS. However, the exact roles of HSP27 in the CNS have not yet been clarified. It has been reported that interleukin (IL)-1β stimulates IL-6 synthesis in C6 glioma cells. In the present study, we investigated the role of HSP27 in the IL-1β-induced IL-6 synthesis in C6 cells. IL-1β alone did not affect the levels of HSP27. The IL-1β-induced IL-6 release in HSP27-downregulated C6 cells were enhanced compared with those in control siRNA-transfected cells. On the other hand, the IL-1β-induced IL-6 release was significantly enhanced in C6 cells transfected with HSP27 than those in control cells in time- and dose-dependent manner. The IL-1β-induced IL-6 release and the mRNA expression were markedly suppressed in C6 cells transfected with phosphorylated HSP27, while those in the cells transfected with unphosphorylated HSP27 were enhanced. In conclusion, these results strongly suggest that phosphorylated status of HSP27 has a switching role in the IL-1β-induced IL-6 synthesis in C6 glioma cells.
热休克蛋白 27（HSP27）是一种低分子量 HSP，被认为是一种分子伴侣。在中枢神经系统（CNS）中，HSP27 的表达会受到各种刺激的诱导。然而，HSP27 在 CNS 中的确切作用尚未阐明。有报道称，白细胞介素（IL）-1β可刺激 C6 神经胶质瘤细胞中 IL-6 的合成。在本研究中，我们研究了 HSP27 在 C6 细胞中 IL-1β诱导的 IL-6 合成中的作用。IL-1β 本身并不影响 HSP27 的水平。与对照 siRNA 转染细胞相比，下调 HSP27 的 C6 细胞中 IL-1β 诱导的 IL-6 释放增强。另一方面，HSP27 转染的 C6 细胞中，IL-1β 诱导的 IL-6 释放呈时间和剂量依赖性显著增强，而对照细胞中则显著增强。IL-1β 诱导的 IL-6 释放和 mRNA 表达在 HSP27 磷酸化转染的 C6 细胞中明显受到抑制，而在未磷酸化 HSP27 转染的细胞中则增强。综上所述，这些结果强烈表明，磷酸化状态的 HSP27 在 C6 神经胶质瘤细胞中 IL-1β 诱导的 IL-6 合成中具有开关作用。
