Department of Pharmacognosy, PES College of Pharmacy, 50 Feet Road, Hanumanthnagar, Bangalore, Karnataka, India.
Phytomedicine. 2011 Jan 15;18(2-3):219-23. doi: 10.1016/j.phymed.2010.07.002. Epub 2010 Aug 22.
Cardiospermum halicacabum roots have been used traditionally for the treatment of epilepsy and anxiety disorders. The purpose of this study was to characterize the putative phytoconstituents present in the ethanolic root extract having anxiolytic activity using an elevated plus-maze (EPM) and light dark transition model. Control mice were orally treated with an equal volume of vehicle (4% gum acacia), and positive control mice were treated with diazepam (1mg/kg). In the EPM test, out of pool of 19 master fractions (MF) only MF-14, 16 and 17 significantly (30mg/kg, p<0.05, p<0.01 and p<0.001) increased the number of entries in the open arm. MF-14, 16 and 17 (10, 20 and 30mg/kg) had also increased the time spent by mice in illuminated part of the box significantly (p<0.05, p<0.01 and p<0.001), as compared to control. However, significant changes (p<0.05, p<0.01 and p<0.001) were recorded in other parameters, e.g., rearing, time spent in the closed arm and dark zone in both the models. These results suggested that C. halicacabum root is an effective anxiolytic agent. The phytoconstituent responsible for the observed central effects was isolated from MF-14 and identified as well-known compound, Cardiospermin, a cyanogenic glucoside.
水瓜柳根传统上被用于治疗癫痫和焦虑症。本研究的目的是使用高架十字迷宫（EPM）和明暗过渡模型来表征具有抗焦虑活性的乙醇根提取物中的潜在植物成分。对照小鼠经口给予等量的载体（4%树胶阿拉伯胶），阳性对照小鼠给予地西泮（1mg/kg）。在 EPM 测试中，在 19 个主馏分（MF）中，只有 MF-14、16 和 17 显著增加了开放臂的进入次数（30mg/kg，p<0.05，p<0.01 和 p<0.001）。MF-14、16 和 17（10、20 和 30mg/kg）还显著增加了小鼠在箱内照明部分的停留时间（p<0.05，p<0.01 和 p<0.001），与对照组相比。然而，在两个模型中，其他参数，如竖起、在封闭臂和暗区花费的时间，都发生了显著变化（p<0.05，p<0.01 和 p<0.001）。这些结果表明，水瓜柳根是一种有效的抗焦虑剂。负责观察到的中枢作用的植物成分是从 MF-14 中分离出来的，并被鉴定为众所周知的化合物，Cardiospermin，一种氰苷葡萄糖苷。
