Uhrig Joachim F, Hülskamp Martin
Botanical Institute III, University of Cologne, Köln, Germany.
Methods Mol Biol. 2010;655:77-88. doi: 10.1007/978-1-60761-765-5_6.
Arabidopsis trichomes are giant single epidermal cells that are easily accessible for genetic, genomic and cell-biological analysis. They have therefore become a convenient model system to study developmental and physiological processes. Trichome studies are greatly facilitated by methods specifically applicable for this particular cell type. In addition, it is very important to use conventions and definitions that have been developed to make studies comparable and capture the relevant aspects. This chapter will highlight these two aspects of trichome analysis.
拟南芥毛状体是巨大的单个表皮细胞,便于进行遗传、基因组和细胞生物学分析。因此,它们已成为研究发育和生理过程的便捷模型系统。专门适用于这种特定细胞类型的方法极大地促进了毛状体的研究。此外,使用已制定的惯例和定义非常重要,以便使研究具有可比性并涵盖相关方面。本章将重点介绍毛状体分析的这两个方面。