Möller-Steinbach Yvonne, Alexandre Cristina, Hennig Lars
Department of Biology, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland.
Methods Mol Biol. 2010;655:229-37. doi: 10.1007/978-1-60761-765-5_15.
A dramatic change in the life cycle of plants is the transition to flowering, which is triggered by both environmental signals, such as temperature and photoperiod, and endogenous stimuli. The dicotyledonous annual plant Arabidopsis thaliana is widely used as a model organism to study how these different signals are integrated into a developmental response. The existence of a diverse collection of Arabidopsis flowering time mutants is particularly useful to understand the genetics of flowering time control. This chapter gives an overview of flowering time analysis, including protocols to measure flowering time in Arabidopsis and wheat. For Arabidopsis, the experimental design necessary to assign flowering time mutants to a specific pathway is described.
植物生命周期中的一个显著变化是向开花的转变,这一转变由温度和光周期等环境信号以及内源刺激共同触发。双子叶一年生植物拟南芥被广泛用作模式生物,以研究这些不同信号如何整合到发育反应中。拟南芥开花时间突变体的多样化集合对于理解开花时间控制的遗传学尤为有用。本章概述了开花时间分析,包括测量拟南芥和小麦开花时间的实验方案。对于拟南芥,描述了将开花时间突变体分配到特定途径所需的实验设计。