Stahl Yvonne, Simon Rüdiger
Institute of Genetics, Heinrich-Heine University Düsseldorf, Düsseldorf, Germany.
Methods Mol Biol. 2010;655:239-51. doi: 10.1007/978-1-60761-765-5_16.
Gene expression can be analyzed at high spatial resolution via RNA in situ detection methods. For many tissues and species, these will be performed on sections of embedded and fixed plant material. When very small or fragile tissues, such as embryos or roots are being investigated, whole mount methods can be employed. Protocols for both approaches are described in detail.
基因表达可通过RNA原位检测方法在高空间分辨率下进行分析。对于许多组织和物种，这些分析将在包埋和固定的植物材料切片上进行。当研究非常小或脆弱的组织，如胚胎或根时，可以采用整装法。两种方法的实验方案都将详细描述。
